RESEARCH INSTRUMENTS 670854 RI Witness Sperm Prep Reader User Manual

RESEARCH INSTRUMENTS LTD RI Witness Sperm Prep Reader

User Manual

User ManualRI Witness™Document 6-70-121UM, Issue 13, 26th January 2015, MRF 1636RESEARCH INSTRUMENTS LTDBickland Industrial Park, Falmouth, Cornwall TR11 4TA, UKt: +44 (0) 1326 372 753 | f: +44 (0) 1326 378 783 |e: sales@research-instruments.comwww.research-instruments.com
CONTENTS  Intended Use 2Medical Device Component 2Applicable Part Numbers 2Compability 3Installaon 3 GuidanceandManufacturer’sDeclaraon(Part15ofFCC) —ElectromagnecEmissions 5GuidanceandManufacturer’sDeclaraon(IEC60601-1-2) —ElectromagnecEmissions 5GuidanceandManufacturer’sDeclaraon —ElectromagnecImmunity 6Safety/InformaonSymbols 8Safety and Reliability 9Temperature Safety 9Mains Power Supply 10RFID Reader Environment 10Startup / Shutdown Procedure 10 Data Capture 12Imaging 12Traceability 13Cryo 13RI Witness™ Work Area 13RI Witness™ Manager for Traceability, Data Capture and Imaging 14RFID Overview 15The Shared RI Witness™ Database 15Hardware Overview 16Work Area Reader Types 16Mulplexer 17Control Units 17
Unheated Control Unit 17Heated Control Unit 18Auto Tuning Readers 18RFCalibraon 19RIWitness™WorkAreaSpecicaonTable 20 Introducon 21RI Witness™ RFID tags 22TaggingPlascware 22Dishes and Pots 22Tubes 22Handling RFID Tags 23How to Switch On 24SystemInformaon 24ToOpentheSystemInformaon 25How to Shut Down a Work Area 25Logging In 25Auto Logon 25Logging Out 26The WorkArea Interface 27Performing A Procedure in RI Witness™ 28Beginning a New Cycle in RI Witness™ 28WitnessPointConrmaon 30Double Witness Points 30QuesonWitnessPoints 31Record Witness Points 32Unassigned Tags 32Tag Mismatch 33Unassigned Tag Removal 33RecordLocaonofTags 34Training Mode 35ClearDemoTables(legacyworkareasforpresowareversion2.3only) 35Admin Assign 36Discards 36Cycle Summary 37CONTENTS
Daily Lists 37Windowed Work Area 37Language and Keyboard Layout 38Foot Pedal Control 38To Rename the Work Area 39ToChangetheLocaonofaWorkArea 39ToViewWorkAreaLocaonsorDisableaWorkArea 40AlarmifTagsLeinWorkArea 40 Logging In 42License Management 42License Request Form 43Database Management 45InialScreen-MainMenuBuons 46Home 46HowtoConguretheHomeScreen 47Paents 48EnteringNewPaentDetailsintoRIWitness™Manager 48EdingExisngPaentDetails 49LookingUpPaentHistories 49Assigning Donor Status 50Assigning Partners 50Cycle Types 51StarngaNewCycle 51Assign a Cycle Type 51ColleconandTransferDates 52Cycle Status 52Revert Status 52RIPaentIdentyLabels 53PrinngPaentLabels 54Label Styles 55Operator Management 55Operator Details 56Group Membership 56Enabled Operators 56Operator PIN 56CONTENTS
Clinic Details 56Mismatch Comments In RI Witness™ Manager 57The Witness Point Diagram 58Links 59Reassigned Tags 59Unassigned Tags 59Witness Point Inputs 60MulpleSourcesoftheSameTagType 60MulpleTagTypes 60Entry Witness Points 60Double Witness Points 60Donor Witness Points 61TaggedDonaons 61UntaggedDonaons 62WitnessPointQuesons 62Witness Point Order 63The Witness Point Log 63Tag Types 63ExploretheWitnessPointLog 64WitnessPointLogStascs 64 AnIntroducontoCryoWitnessing 65PrinngBarcodes 65Scanning Barcodes 67 Managing Materials 68Material Types 68Material Type Details 69Notes on GS1 Barcodes 70Cycle Types 70CreangaNewBatch 70DelengaBatch 72Batch Status 72ExpiredBatches 73View All Batches 74CONTENTS
Paents-ManagingTreatmentCycles 74ExcludingandIncludingBatchesforaCycle 74 AnIntroducontoDataCaptureforDataCollecon 75Collected Data Setup 75ParameterConguraon 76Numerical Parameters 76TextParameters 76List Parameters 77Check Parameters 77Date, Time and Date Time Parameters 78CalculaonParameters 78Built In Parameters 78Sheet Contents 78Shared Between Sheets 79Treatment Cycle Sheets 80ViewingandEdingSheets 80EdingSheetsattheWorkAreaTouchScreen 80ViewingandEdingSheetswithRIWitness™Manager 82Egg Parameters 82The Number of Eggs 84Egg Count Parameter 84EggCountConict 84Sheets and Witness Points 86Cycle Summary Sheets 86ParameterAributes 87InialValue 87Editable 88Required 88 How to View Live Images 89MulpleScreenConguraon 89How to Take a Picture 90How to Record Video 91How to Zoom & Pan the Image 91CONTENTS
How to Perform Measurements 91How to Select Cameras 92Camera Flipping 92How to Set the Preset Zoom 92HowtoConguretheFootPedal/Keyboard 92HowtoSelectObjecves 92HowtoAddObjecves 93HowtoRemoveObjecves 93HowtoCheckObjecveCalibraon 93PaentDisplay 93Use of Saturn™ Laser Systems with RI Witness™ 94HowtoAccessSaturnLaserSystemInformaonfromaWorkArea 94  TemperatureProle 97TemperatureProleChecklist 97TemperatureCalibraon 97TemperatureCalibraonService 98TouchScreenCalibraonChecklist 99 Cleaning 100 Reuse Statement 101RI Repairs System 101Product Disposal (European Union) 101RI Returns System 101Contact Details 101ObligaontoInform 101Feedback 102CONTENTS
Secon 1RI Witness™ RFID Tags11Thank you for choosing RI Witness™.The RI Witness™ family of products is made up of RI Witness™, Data Capture, Traceability, Imaging and Cryo.ThismanualprovidesallnecessaryinformaontouseRIWitness™.Thesystemshouldbeoperatedbytrainedpersonnelonly.Allseconsofthismanualshouldbereadandunderstoodfullybeforeanyoperaonofthesystem.PleaseseetheIntendedUseformoreinformaon.IftheoperatorisunsureofanyoftheinformaoncontainedinthismanualtheyshouldcontactResearchInstrumentsoranappointedrepresentavebeforeaempngtousethisequipment.In no event does Research Instruments Ltd (RI) assume the liability for any technical or editorial errors ofcommission,oromission;norisRIliablefordirect,indirect,incidental,orconsequenaldamagesarising out of the use or inability to use this manual.The informaon in this manual is current at the me of publicaon. Our commitment toproduct improvement requires that we reserve the right to change equipment, procedures and specicaons at any me. The latest version of the User Manual can be downloaded from soware.research-instruments.com.TheRI Witness™ manual belongs with the RI Witness™systemand should be passed on with the system if relocated to another clinic.The use of ™ in this manual indicates a trademark of Research Instruments Ltd. Any other brand names,referredtointhismanual,aretrademarksoftheirrespecveowners.© This manual is protected by copyright, all rights reserved, and no part here of may be photocopied orreproducedinanyformwithoutthepriorwrienconsentofRI.             
Secon 22Introducon2For RI Witness™ heated work areas only.Toidenfyandtrackhumansamplesthroughtheassistedreproducon(AR)cycleandwhererequired,to maintain sample temperature. Therearenocontraindicaonsassociatedwiththeuseofthisdevice.Theheatedplate(andtemperaturecontrol)componentsofthissystemareclassiedasaclassIImedicaldeviceinaccordancewithArcle9,AnnexIX,rule9oftheDirecves93/42/EEC&2007/47/EC,ieit‘administersorexchangesenergy’toorfromthehumanbody.InrespectoftheRIWitness™system,energyisemiedintheformofheat,toorfromtheARsample.Thereisnodirectpaentcontact. Applicable indicaons for use are subject to the regulaons of the country into which the device is sold. Availability of RI Witness™ for clinical use is dependent on the regulatory approval status of RI Witness™ within the country the device is intended to be sold into. 6-70-801 Sit on Top Heated Work Area Assembly6-70-801/C Sit on Top Heated Work Area Assembly with Card Reader6-70-801/T Sit on Top Heated Work Area Assembly with Test Tube Reader6-70-801/TC Sit on Top Heated Work Area Assembly with Card and Test Tube Reader6-70-802 FlushFiedHeatedWorkArea1ChannelAssembly6-70-802/C FlushFiedHeatedWorkArea1ChannelAssemblywithCardReader6-70-802/T FlushFiedHeatedWorkArea1ChannelAssemblywithTestTubeReader6-70-802/TC FlushFiedHeatedWorkArea1ChannelAssemblywithCardandTestTubeReader6-70-803 Slim Heated Work Area Assembly6-70-803/C Slim Heated Work Area Assembly with Card Reader6-70-803/T Slim Heated Work Area Assembly with Test Tube Reader6-70-803/TC Slim Heated Work Area Assembly with Card and Test Tube Reader6-70-804 FlushFiedHeatedWorkArea2ChannelAssembly6-70-804/C FlushFiedHeatedWorkArea2ChannelAssemblywithCardReader6-70-804/T FlushFiedHeatedWorkArea2ChannelAssemblywithTestTubeReader6-70-804/TC FlushFiedHeatedWorkArea2ChannelAssemblywithCardandTestTubeReader
Secon 2Introducon32RIWitness™isusedinconjunconwiththefollowing:• Essenalmedicaldevices-dishesandtubes,maybeARornot-ARspecic.• Nonessenalmedicaldevices-safetycabinets,incubators,micromanipulators,lasers.• Non medical devices (general laboratory equipment), eg work benches, microscopes, PCs.InstallaonsofRIWitness™shouldbecarriedoutbyaRItechnicianorotherRIauthorisedpersonnel.Incorrectinstallaoncouldresultinoverallpoorperformance. 6-70-805 ITO Reader6-70-805/C ITO Reader with Card Reader6-70-805/T ITO Reader with Test Tube Reader6-70-805/TC ITO Reader with Card Reader and Test Tube Reader6-70-806 Sit on Top Heated Work Area 2 Channel Assembly6-70-806/C Sit on Top Heated Work Area 2 Channel Assembly with Card Reader6-70-806/T Sit on Top Heated Work Area 2 Channel Assembly with Test Tube Reader6-70-806/TC Sit on Top Heated Work Area 2 Channel Assembly with Card and Test Tube Reader6-70-852 SpermPreparaonWorkAreaAssembly6-70-852/C SpermPreparaonWorkAreaAssemblywithCardReader6-70-853 Unheated Work Area Assembly6-70-853/C Unheated Work Area Assembly with Card Reader6-70-853/T Unheated Work Area Assembly with Test Tube Reader6-70-853/TC Unheated Work Area Assembly with Card and Test Tube Reader
Secon 3Safety Warnings3disassembleormodifyanypartoftheRIWitness™,orsubstuteanycomponentfor any other. Doing so may result in damage to samples. This voids the warranty and/or service contract. To avoid the risk of electric shock, this equipment must only be connected to a supplymainswithprotecveearth. use the power cable and power supply adaptor supplied with the system.The cable to the power supply is the ‘disconnect device’ for this equipment. To remove all electrical power from this product, disconnect the power cable from the electrical outlet. Equipmentshouldbeposionedsoastoalloweasyaccesstothepowercable.Theappliancecoupler or mains plug is used as the disconnect and must remain readily operable.Nottobeusedinapaentenvironment. Thesystemshouldbeoperatedbyqualiedandtrainedpersonnelonly.Thissymbolindicatescauonarytextwhichshouldbefollowedtoavoidinjurytousersordamage to samples.
Secon 3Safety Warnings53RIWitness™isintendedforuseintheelectromagnecenvironmentspeciedbelow.Thecustomerorthe user of RI Witness™ should ensure that it is used in such an environment.   RF emissionsCISPR 11 Group 2RI Witness™ must emit electromagnec energyinordertoperformitsintendedfuncon.Nearbyelectronicequipmentmaybeaected.RF emissionsCISPR 11 Class BRI Witness™ is suitable for use in all establishments otherthandomescandthosedirectlyconnectedto the public low-voltage power supply network that supplies buildings used for domescpurposes.Harmonic emissionsIEC 61000-3-2 Not applicableVoltageuctuaons/ickeremissionsIEC 61000-3-3Not applicable  This equipment has been tested and found to comply with the limits for a Class A digital device, pursuanttopart15oftheFCCRules.Theselimitsaredesignedtoprovidereasonableproteconagainstharmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance withtheinstruconmanual,maycauseharmfulinterferencetoradiocommunicaons.Operaonofthisequipmentinaresidenalareaislikelytocauseharmfulinterferenceinwhichcasetheuserwillberequiredtocorrecttheinterferenceathisownexpense.ThisdevicecomplieswithIndustryCanada’slicence-exemptRSSs.Operaon issubject tothefollowingtwocondions:1. This device may not cause interference.2. Thisdevicemustacceptanyinterference,includinginterferencethatmaycauseundesiredoperaonof the device.
Secon 36Safety Warnings3 IEC 60601  Electrostacdischarge(ESD)IEC 61000-4-2± 6 kV contact± 8 kV air± 6 kV contact± 8 kV airFloors should be wood, concreteorceramicle.Ifoorsarecoveredwithsynthecmaterial,therelavehumidityshould be at least 30 %.Electrical fast transient/burstIEC 61000-4-4± 2 kV for power supply lines± 1 kV for input/output lines± 2 kV for power supply lines ± 1 kV for input/output LinesMains power quality should be that of a typical commercial or hospital environment.SurgeIEC 61000-4-5± 1 kV line(s) to line(s) ± 2 kV line(s) to earth±1kVdierenal Mode ± 2 kV common modeMains power quality should be that of a typical commercial or hospital environment.Voltage dips, short interruponsandvoltagevariaonsonpowersupply input linesIEC 61000-4-11<5 % UT (>95 % dip in UT ) for 0.5 cycle 40 % UT (60 % dip in UT) for 5 cycles 70 % UT (30 % dip in UT) for 25 cycles <5 % UT (>95 % dip in UT) for 5s<5 % UT (>95 % dip in UT) for 0.5 cycle 40 % UT (60 % dip in UT) for 5 cycles 70 % UT (30 % dip in UT) for 25 cycles <5 % UT (>95 % dip in UT) for 5sMains power quality should be that of a typical commercial or hospital environment. If the user of RI Witness™ requiresconnuedoperaonduringpowermainsinterrupons,itis recommended that RI Witness™ be powered fromanuninterrupblepower supply or a baery.Power frequency(50/60 Hz)magneceldIEC 61000-4-83A/m 3A/m Power frequency magneceldsshould be at levels characteriscofatypicallocaoninatypical commercial or hospital environment.UTisthea.c.mainsvoltagepriortoapplicaonofthetestlevel.
Secon 3Safety Warnings73 IEC 60601 Conducted RF IEC 61000-4-6Radiated RF IEC 61000-4-33 Vrms150 kHz to 80 MHz3 V/m80 MHz to 2.5 GHz3 Vrms3 V/mPortableandmobileRFcommunicaonsequipment should be used no closer to any part of RI Witness™, including cables, than the recommended separaondistance calculated from the equaonapplicable to the frequency of the transmier.Recommendedseparaondistanced = [3.5/V 1]√pd = [3.5/V1]√p80MHz to 800MHzd = [3.5/V1√p800MHz to 2.5GHzwhere p is the maximum outputpower rang of the transmier inwas (W) according to the transmiermanufacturer and d is the recommended separaon distancein metres(m). Fieldstrengths from xed RF transmiers,as determined by an electromagnecsite survey, a should be less than the compliance level in each frequency range.b Interference may occur in the vicinity of equipment marked with the following symbol: At 80 MHz and 800 MHz, the higher frequency range applies.Theseguidelinesmaynotapplyinallsituaons.Electromagnecpropagaonisaectedbyabsorponandreeconfromstructures,objectsandpeople.Theseguidelinesmaynotapplyinallsituaons.Electromagnecpropagaonisaectedbyabsorponandreeconfromstructures,objectsandpeople.aFieldstrengthsfromxedtransmiers,suchasbasestaonsforradio(cellular/cordless)telephonesand land mobile radios, amateur radio, AM and FM radio broadcast and TV broadcast cannot be predicted theorecallywithaccuracy.ToassesstheelectromagnecenvironmentduetoxedRFtransmiers,anelectromagnecsitesurveyshouldbeconsidered.IfthemeasuredeldstrengthinthelocaoninwhichRIWitness™isusedexceedstheapplicableRFcompliancelevelabove,RIWitness™shouldbeobservedtoverifynormaloperaon.Ifabnormalperformanceisobserved,addionalmeasuresmaybenecessary,suchasre-orienngorrelocangRIWitness™.bOverthefrequencyrange150kHzto80MHz,eldstrengthsshouldbelessthan[V]V/m.
Secon 38Safety Warnings3 Indicatesinstruconfordisposalofgoods.In accordance with Annex II of the EuropeanMedicalDeviceDirecve93/42/EEC,asamendedbyDirecve2007/47/ECunderthesupervisionofnoedbodyNo.0120,SGS,UKLtd.In accordance with the European Direcve forR&TTE,Direcve1999/5/ECIndicates the medical device manufacturer.Indicates the need for the user to consult the instrucons for use for important cauonaryinformaonsuchaswarningsandprecauonsthatcannot, for a variety of reasons, be presented on the medical device itself.Followinstruconsforuse.Therstfourdigitsareauniqueidenerassignedto the product and the last 2 digits signify the year of manufacture, eg 5001/13 (this denotes a unique serial number of 5001 and a year of manufacture of 2013).Cauon: US Federal law restricts this device forsale to or on the order of a licensed healthcare praconer.SNOnly
Secon 3Safety Warnings93Pleasereadthismanualcarefullyandfollowtheinstruconstoensurethatthesystemwillworksafelyand reliably.Safetyistheresponsibilityofthelaboratory.Riskassessmentandworkingpraccesshouldcomplywithlocal regulatory policies.A warning triangle will be displayed on the work area touch screen and on the control unit display if the currently selected temperature cannot be maintained.A touch screen warning triangle will also be shown if temperature control has been disabled using the TurnObuon.Gently place your hand on the heated surface to verify that the temperature is appropriate for use.Aswithallheangsystems,itisadvisabletoperformaperiodiccheckoftemperaturesusingacalibratedthermocouple thermometer.
Secon 310Safety Warnings3 An RI Witness™ system uses readers to monitor a work area. Readers detect RFID tagged containers that are placed in the work area.Eachreaderisindividuallytunedtoitsenvironmentandmustnotbereposionedaerinstallaon.TheperformanceofRFIDtagdeteconmaybecompromisedbyreposioningreadersandbytheproximityofmetalobjectsorelectricalequipmentthatwerenotpresentduringinstallaon.Forcleaning,readersmaybeliedandreturnedtothesameposion.See“Cleaning”onpage100formore cleaning details.    RI Witness™ hardware may be damaged by incorrect startup and shutdown procedures.“Secon5-RIWitness™Basicoperaon”describestherecommendedstartupandshutdownprocedurefor the RI Witness™ work area.Products from RI require a stable and noise free power supply ( 100V - 240V ). The supply must provide anearthconnecon.The RI Witness™ control unit for heated readers contains a mains power supply.
Secon 4Product Overview114Welcome to the User Manual for the Research Instruments (RI) RI Witness™ System. RI Witness™, Data Capture, Traceability, Imaging and Cryo are members of the RI Witness™ family of products. RI Witness™ products share a common database usually referred to as the “Witness”database.RIWitness™isasystemwhichoperateswithinanassistedreproducon(AR)clinicsengandprovidesamethodofidenfyinghumansamplesthroughoutan AR cycle(fromegg and spermcollecontoembryo transfer). The system is intended to minimise the risks associated with tradional/manualdouble-checkingandprovidestheessenalcontrolsnecessarytoensureeggs,spermandembryosarecorrectly matched and treated during the AR process.The RI Witness™ system comprises hardware, rmware and soware components, which can becongureddependingonthetreatmentacvies,numberofARcyclesconducted,sizeandlayoutofthe AR clinic.RFID (radio frequency idencaon) technology provides the means of idenfying the containers(dishes, tubes) in which eggs, sperm and embryos are transferred and stored. The containers are labelledbyaclinicianwithaspecialRFIDtagwhichhasbeenassignedauniqueidener.Theuniqueidenerislinkedtoapaent/couple(specicparentage).As samples are processed as part of an AR cycle, RFID readers (both heated and non-heated) read the tagson the containerand their identyand status is conrmedon-screen.If containers containingsamplesofincompableorigincomeintocontactatanystageofthisprocess,thesystemacvatesanalarm and prompts the clinician to respond.Figure 4-1 RFID tag communicang with various antennasAn antenna is incorporated into a work area reader. A control unit feeds an RF signal to the antenna. An RFIDtagispassiveunlenergisedbythesignalfromtheantenna.Theenergisedtagthentransmitsanidencaoncodebacktothesameantenna.SeeFigure4-1.AnARprocedureisconductedwithinthemonitoredworkarea.Theprocedureisdenedbyasequenceof Witness Points which are presented on the touchscreen.RI WitnessComputer Control UnitSit on Top Heated Work Area ReaderRFID Test Tube ReaderRFID TagMulplexerTouch Screen
Secon 412 Product Overview4Hand wrien notes are oen taken whilst performing laboratory procedures. For example embryoscoresandspermvolumesmightbewriendownonadatasheetandlatermanuallytranscribedintoaclinicferlitydatabase.The features of Data Capture allow data sheets to be designed in RI Witness™ Manager and data entry to be performed in the laboratory using the work area touch screen. See Figure 4-2.Data entry may also be performed in RI Witness™ Manager.Figure 4-2 Lower: Data entry using a RI Witness™ WorkArea touchscreen Upper: Data sheet design using RI Witness™ Manager With the Imaging feature, you are able to capture images and videos from every microscope in the laboratory,ateverystageofthepaentcycle,inrealme.Allthelatestinformaononacyclecanbeaccessed immediately from any networked PC. Images can also be sent to the embryo room to show thepaentpriortoembryotransfer.TheImagingfeatureiscompablewithRIIntegra™andSaturn™lasers.Figure 4-3 Example of paent view shown in embryo room
Secon 4Product Overview134Traceabilityisasowareproductthatlinkspaenttreatmentcycleswiththebatchesofmaterialsthatare in use.A barcode reader may be used to scan batches as they are delivered or made ready for use.Thelinksbetweentreatmentcyclesandmaterialbatchesmaybeexploredtogeneratevariousreports,forexampleareportshowingallpaentsthathavebeenexposedtoaparcularmaterialbatch.TheCryofeatureextendstheRIWitness™securitysystem,allowingpaentsamplestobetrackedastheyenterandleavecryostorage,creangacompleterecordofthepaentcycle.A barcode reader is placed at the work area and used to scan a sample in or out of the cryo storage.Figure 4-4 An RI Witness™ work area RFID tagged items are displayed on a touchscreen running RI Witness™ WorkArea soware.A typical RI Witness™ work area includes a PC, touchscreen, one or more tag readers and an RF control unit.TheworkareaPCrunsRIWitness™WorkAreasowaretopresentanoperatorinterfaceonthetouch screen. See Figure 4-4.The data presented on the touchscreen is generated from the shared Witness database, all work area events are logged to the shared Witness database.
Secon 4 Product Overview4RI Witness™ Manager runs on any Windows PC and provides Witness, Data Capture, Cryo, Traceability andImagingfunconality.The“paents”RIWitness™Managerpageisusedbyalloperators,the“materials”RIWitness™ManagerpageisuniquetoTraceabilityoperatorsandthe“witnesspoints”pageisuniquetoWitnessoperators.See Figure 4-5.Figure 4-5 RI Witness™ Manager is used by RI Witness™, Data Capture, Traceability, Imaging and Cryo
Secon 4Product Overview154AnexampleRIWitness™installaonisshowninFigure4-6.FourworkareaswithRIWitness™WorkAreasoware, two RI Witness™ Manager PCs and an admin reader PC share a common RI Witness™database.Traceability, RI Witness™ and DataCapturemanagementfuncons maybe performedateach RI Witness™ Manager PC.LabManagerWork AreaWork AreaShared RI Witness™ DatabaseLabManagerAdmin ReaderWork AreaWork AreaFigure 4-6 The RI Witness™ database is shared by All RI Witness™ WorkArea and RI Witness™ Manager PCsRadio-frequency idencaon (RFID) is the technology used by RI Witness™ to idenfy taggedplascware.AcontrolunitfeedsanRFsignaltoamatchedantenna.AnRFIDtagispassiveunlenergisedbythesignalfromtheantenna.The energisedtagthentransmitsanidencaon codebacktothe sameantenna. See Figure 4-1.AnRFIDsystemworksontheprincipleofinducvecoupling,whichrequiresthattheantennabetunedtomatchthefrequencyinuseandopmisedforthephysicalenvironment.SpeciccablelengthsmustbeusedforallRFconnecons.EachRIWitness™workarearequiresacomputer,acontrolunitandatleastoneantenna.MulpleantennaworkareasrequireamulplexertoswitchtheRFsignalbetweenantennas.Antennas are built into RI Witness™ Readers.
Secon 416 Product Overview4ReadersdetectRFID(RadioFrequencyIdencaon)tagsusingtunedantennas.Thetesttubereader (2axis)andthespermpreparaonreader(3axis)useonetunedantennaforeachaxis.Allotherreadersuse a single tuned antenna.Aushngvariantoftheheatedreaderisavailable,thisisintegratedintothesurfaceofthesafetycabinetforeaseofuse.Legscanbeedtoallowfurtherintegraonwithotherlightbases.Figure 4-7 ITO reader Figure 4-8 Card reader Figure 4-9 Admin readerFigure 4-10 Sit on top heated reader Figure 4-11 Sit on top unheated readerFigure 4-12 RFID Test tube reader Figure 4-13 Sperm preparaon reader
Secon 4Product Overview174AmulplexerdistributesRFpowertomulpleantennas.Amulplexercanfeeduptofourantennas.Control units provide the RF power for readers. They may be used for heated or non-heated work areas depending on the procedures conducted there.Figure 4-14 An RI mulplexerTheunheatedcontrolunitmaybeusedwithamulplexertodriveuptofourantennas. Mains socket  USB to PC RFCoaxialOUTtomulplexerorheatedreaderFigure 4-15 Back panel of unheated control unit1 32
Secon 418 Product Overview4Theheatedcontrolunitcontainsatemperaturecontrollerandmaybeusedwithamulplexertodriveup to four antennas.1 2 3 41. RFCoaxialOUTtomulplexerorheatedreader2. USB to PC3. Heater cable to reader4. Mains socketFigure 4-16 Back panel of heated control unit1. Place the reader in the desired area2. Remove the cable clamp3. Connect the power supply4. Connect the Mini-USB connector5. Replacetheretainingbracketandghtenscrew6. Connect the USB-A connector to the PC, Tablet or USB ConneconsaremadeupofaUSBA-Bcableandapowersupply.Thepowersupplyisa12Vpowersupplyandisexternaltothedevice.TheUSBisastandardUSB2.0cable.Thesearelocatedontherearof the device and held in place by a retaining bracket and screw. Figure 4-17 Sperm preparaon reader
Secon 4Product Overview194CalibraonoftheRIWitness™SpermPrepReaderismadealotsimplerbyhavinganautomactuningsystem. The procedure for this is as follows.1. OpenRIWitness™WorkArea.MakesuretheRFIDreaderandMulplexeraredetected.2. Click the buonontheboomrightofthiswindow.3. FromthisyouwillseetheWorkareaSengswindow.ClickontheRFIDReadingtabtobringuptheRFIDConguraonWindow.Figure 4-18 Figure 4-19 4. FortheSpermPrep,settheAntennaConguraonto3.Thenextthingtodoistoclickon.Thiswilltunetheantennasfortheareatheyaresituatedin.Everymethedeviceismoved this will need to be done. This will take a few moments but when done the SWR for each channel should show a value of 1-50. If there are any RI tags in the work area, then they will be displayedinthegreybox.YouarenowreadytousetheRIWitness™SpermPrepReader.
Secon 420 Product Overview4 HeangSystems* Heated metal and glass.TemperatureSensor* Digital 14-bit SHT-15 or Analogue PT1000.Displays* LCD Display. Accurate to 1 decimal place.Connecvity USBTypeBsocketforconnecontoPC.ConnectedPCtobecompliantwith IEC 60950-1.Supply Voltage 100-240VAC,50-60Hz,Max.2.6A,ClassI.OperangTemperature* Temperature: 10°C (50°F) to 42°C (107.6°F).Humidity: 15% to 85% RH (Non Condensing).HeaterChannels* 2Channel122VDCat6AMax.Channel222VDCat1Amax.RFID 50ΩLoadat13.56Mhz1.4WMax.I-4Antennas.*Forheatedworkareasonly
Secon 5RI Witness™ Basic Operaon215RI Witness™ uses one or more readers to monitor RFID tagged containers in a work area.The work area interface is presented on a touch screen. See Figure 5-1. The operator touches screen buonstologinandselectWitnessPoints.Figure 5-1 The RI Witness™ WorkArea interface is presented on a touch screen
Secon 522RI Witness™ Basic Operaon5Beforeuseintheworkarea,allplascwaremustbeRFIDtaggedandlabelledwithpaentidenty.Rectangular,circularandsquaretagsareavailable.SeeFigure5-2.ThesampleidentymaybeassignedtotheRFIDtagoncethetaggedplascwareisplacedinaRIWitness™workarea.RFIDtagsmustbeposionedonthebaseofdishesandpots.Thesquaretag,posioneddiagonally,isrecommended for a four well dish.Figure 5-2 RI Witness™ tags            Therectangulartagisrecommendedfortubes.Posionthelongedgeofthetagalongthelengthofthetubeandholdinplaceusingtapeorapaentidentylabel.Posiontagsnearthetopoftubestoensure they are not obscured by thermal blocks or tube warmers.Rectangular TagSquare TagCircular TagDia 43.8mm BridgeChipPeel from here36 mm18 mmBridgeChip+ UPMPeel from here18 mm18 mmBridgeChip+ UPMPeel from here375_1 +22 mm12 mmPeel from hereChipBridgeTime-Lapse Tag
Secon 5RI Witness™ Basic Operaon235When removing tags, bend the backing strip away from the tag, rather than the tag away from the backing strip. This will reduce the risk of damaging tags. 1. Startpeelingthebackingstripawayatthe“peelfromhere”pointshowninFigure5-3toachievea peel line as shown. chip and bridge line peel lineFigure 5-3Figure 5-4 2. Whensckingthetagtothedish,bringthetagintocontactwiththedishatonepoint.Thenuseyourngerorthumbtoworkthetagontothedishwithacircularmovement,movingawayfromtheinialcontactpointsoastoavoidanykinksinthetagasitsckstothedish.SeeFigure5-4.Unusedtagsandunusedtaggedplascwareshouldnotbestoredonornearasurface(safetycabinetor workbench) where a RI Witness™ reader is located.
Secon 5RI Witness™ Basic Operaon5RI suggests that you keep the RI Witness™ computers and work areas switched on. This means that the heangandmonitoringisconstant.IfyouhaveswitchedoaheatedRIWitness™workarea,pleaseallow45minutesfortheworkareatoreturn to the required temperature. Please check there is power at the wall socket, the PC and touch screen (or tablet) and control tower.You may want to check the work area heang and touch screen calibraon for accuracy beforecommencingwork.See“Secon12-TemperatureCalibraon”onpage97.Thesysteminformaonpanelwillshow:1. Versionnumbersforsowareandrmware2. Workarealocaon3. Database version4. Licensing status including Imaging dongle5. RFIDreaderstatusandmulplexer6. Temperature control status7. Saturn™LaserSystemstatus,serialnumber,rmwaredetails8. Camera status9. Integra™ status61235789Figure 5-5
Secon 5RI Witness™ Basic Operaon255Figure 5-6 RI Witness™ WorkArea icon1. Press the  icon in the work area or click the  icon from the Imaging screen.2. TheSystemInformaonwindowshowsthecurrentstateoftheRIWitness™system.3. Indicates there is no problem. 4. Indicates a problem needs resolving.5. InsystemInformaon,clickingontheclosed attheboomofthepagewillunlockalltheexisngconguraonsengsandalsorevealthefollowingsengsthatarehiddenwhentheSystemInformaonislocked:• Keyboard / foot pedal controls• Auto logon• Language• ScreenconguraonTheSystemInformaonislockedbydefaultandclickingontheagainwillre-lockthesengs.DonotswitchoanyRIhardwarewhilsttheWorkAreasowareisrunning.Tologout,selecttheusername, and then select  in the user name window. The system will then shut down. See Figure 5-8.ItisimportanttoensurethatallRIWitness™hardwareisswitchedonbeforetheworkareasowareisacvated.TheRIWitness™sowaremaybeacvatedautomacallywhenthecomputerisstarted.Ifnot,doubleclick the  desktop icon. To log in either touch  then your username or scan your operator ID tag and enter the appropriate 4 digit PIN if requested.Operators can change the PIN assigned by the administrator and may assign an ID tag. See Figure 5-9.Aerunlockingthesysteminformaon,screennavigatetotheautologonsecon.Thereyoucansetupthe username and password (of the machine) for RI Witness™ to auto logon the PC.1. Click 2. Unlocksengs.3. Click 4. Click  to the user  message.5. Enter the details required (contact IT department if unsure).6. Click 7. Tick 8. Click the close window cross.Oninstallaonoftheapplicaontheapplicaonshortcutisplacedinthestart-up.Thiscausestheworkareatoautomacallystartwithwindows.Figure 5-7 AutoLogon
Secon 526RI Witness™ Basic Operaon5Logoutoftheworkareawhenyouhavenishedyourproceduresbypressingyour in the top right corner, then the  .Anautomaclogoutmaybeprogrammedaeraperiodofinacvity.ThisissetusingtheRIWitness™ManagerSengspage.Figure 5-9 RI Witness™ WorkArea logon and PIN entry windowsFigure 5-8 Logging into RI Witness™
Secon 5RI Witness™ Basic Operaon275The WorkArea interface screen is divided into 7 regions. See Figure 5-10 .   Thisregionshowsthecurrentpaentname,dateofbirthandID.Whentagsfrombothpartnersarepresentthenameshownwillbethefemalepaent.Thepartner’snamewillappearinbrackets.  Therecenthistoryregionpresentsthefourmostrecentaconsthathavebeenrecordedagainstthecurrentpaent,egwitnesspoints(acon/protocolstep),operatormismatches,etc.Amestampandoperatornamearealsoshown.  The work area region displays an icon for each RFID tag detected by the work area. As tags progress through the witness points of a procedure they are assigned and reassigned appropriately.AquesonmarkiconwillbeshownforanytagsthathaveyettobeassignedanidentybytheRIWitness™system.  The status region summarises the work area contents. It is recommended that the number of tagsdisplayedinthestatusareaisvisuallyconrmedateachstageofaproceduretoverifythateach tag has been detected.   For heated systems this region displays the temperature of the work area. Click the temperature readout to show a  window from where the temperature may be set. EmilyFigure 5-10 The WorkArea interface123567
Secon 528RI Witness™ Basic Operaon5  Thewitnesspointsregiondisplaysoponsavailableforthenextstepasspeciedbythelabdenedprotocol.Thetoponeistheprecedingaconandthecurrentcontentsoftheworkareadeterminewhatnextstepsarepossible.Anadministratorwillalsoseeanopontoassign or reassign dishes outside of the prescribed protocol.  Thecurrentoperatorregionshowswhoisloggedon.Asux(Admin)isaddedforoperatorswhobelongtotheadministratorgroup.Allwitnesspointsperformedwillbeaributedtotheoperatorwhoisloggedinatthatme. Bringthedishortubeyouwishtoworkonintotheworkarea.Ifthetaghas beenidenedbyRIWitness™thepaentandthehistorywillbeshownonthescreen.Theoponsforthenextaconswillbe shown in yellow on the witness point region. Intheexampleshownbelowa“SpermWashTube”andan“EggCultureDish”havebeenplacedintothe work area and RI Witness™ has determined that the only appropriate procedure is represented by the  witness point. See Figure 5-11.Figure 5-11 The IVF inseminaon witness point is the only match for the contents of this work areaTherststepintheRIWitness™procedureiswhentheidentycardforthepaentisassigned.Thisisthemostimportantstepintheprotocolasiftheidentyofthepaentiscorrectlyassignedatthispoint, the security of the cycle is assured.
Secon 5RI Witness™ Basic Operaon295Figure 5-12 Unassigned tag has been detected Figure 5-13 Choose Paent/All Paents window to assign ID1. To start a new procedure, log in then place one or more unassigned, tagged items into the work area(usuallyapaentIDcard,eggcollecondishorspermpot).Ayellowquesonmarkwillshow that the tag has been detected and is unassigned. See Figure 5-12. Press the icon.2. In the tabtypethepaentnameorIDnumbertolocatethecorrectpaentinthedatabase. See Figure 5-13.3. Alternavely,thetabshowsallEggColleconsandEmbryoTransfersscheduledfortheday.ClickthepaentcycleforeitherEggColleconorEmbryoTransfer,thiswilldisplaythechosenpaentsdetailsintheworkarea.4. Choosewhichaconyouintendtoregister/performandthenclickthe icon again.5. If a double check by another person has been set at this point, a 2nd Witness window will appear.ThisrequiresacolleaguetoverifytheaconbyinpungtheirnameandPIN.See“DoubleWitnessPoints”onpage30.
Secon 530RI Witness™ Basic Operaon5Ateverystepintheprocedure,awitnesspoint(acon)mustberegisteredinthesystem.Thereforewhenanaconisperformed,selecttherelevantwitnesspointandconrmbycheckingthedetailsintheconrmaonwindowandclickingthe icon . Figure 5-14 Conrming a witness pointFigure 5-15 A double witness pointCricalpoints,asdenedbytheadministrator,mustbewitnessedbyasecondoperator.Thesecondwitness must enter their PIN. See Figure 5-15.
Secon 5RI Witness™ Basic Operaon315Queson Witness Points, as dened by the administrator, require a response from the operator.ExamplesoftextandnumericquesonsareshowninFigure5-16andFigure5-17.The Witness Point queson and the operator response is displayed during the nal witness pointconrmaonwindow.Thequesonandresponsealsoformpartofthehistoryloggedforthisevent.Figure 5-16 Add witness point quesonFigure 5-17 Edit witness point queson
Secon 532RI Witness™ Basic Operaon5Thetagsonunusedplascwareareunassignedandareshownasaquesonmarkiconintheworkareadisplay. SeeFigure5-18.Whenselecngawitnesspointunassignedtagsareautomacallyassignedtothepaentcurrentlydisplayedonthescreen.Theywillbeidenedasthesampletagdenedbythewitness point.Figure 5-18 The queson mark icon represents an unassigned tagGatherallthe(tagged)plascwarethatarerequiredtoperformaprocedureandplacetheminthework area. Awindowrepresenngtheworkareawilldisplayallthetagsandshowtheidentyofanytagsalready“known”tothesystem.An acve paent is selected either manually or automacally; manually by clicking on the seconoftheworkareaandchoosingapaentfromthelist,orbythesystemautomacallyselecngthepaentidenedbythetagsintheworkarea:• Ifthereisonepaentintheworkareathenthisisthecurrentpaent.• Ifthereare2paentsintheworkareawhoaremale/femalepartnersthenthefemalepaentisthecurrentpaent.• Ifthereare2ormorepaentsaeradonaonwherethepaentsaredonor/recipient(s)thenthedonoristhecurrentpaent.Alistofpossiblewitnesspointsisshown,determinedbytheselectedpaentandvisibletags.Choosethe appropriate witness point.Ifthewitnesspointisataggeddonaontype,thentherecipientwillneedtobechosenfromalistatthis point.Ifthewitnesspointrequiresfurtherinformaonenteredbytheoperator,thenthiswillbeaskedfor.If the witness point requires a 2nd witness, then another operator will now need to choose their name and enter their PIN.Aconrmaonwindowsummarisesallthedetailsrelangtothewitnesspointthatwillberecorded.Ifallthedetailsarecorrect,thenselecngtheckwillrecordthewitnesspoint.If any new tags are added to the work area during the processing of a witness point (or admin assign) then the witness point will be cancelled. Also, if during the processing of a witness point one of the tags in the work area drops out of range of the reader(s), then the witness point can be completed and the tag(s) will be reassigned successfully.
Secon 5RI Witness™ Basic Operaon335Figure 5-19 Unassigned tag removalA  warning is displayed when an unassigned tag is removed from the work area. See Figure 5-19. Thismessageinformstheoperatorthatanunassignedtagenteredandexitedtheworkareawithoutbeing registered in the current procedure. If that is correct selectandconnue.Ifnot,replacethe sample/labware in the work area and perform a witness point.TheRIWitness™systemwillonlyallowonepaent(withdonor)oracouple’ssamplestobeplacedinaworkareaatanyme.Ifanunlinkedsampleisplacedintheworkarea,aconnuousalarmwillsoundanda mismatch window will appear immediately. The  message is displayed.Figure 5-20 Tag mismatch1. Remove the samples in order to stop the alarm and select  to close the window. 2. Amismatchreasonwindowisdisplayed.Theoperatormustenteranexplanaonforthemismatchbeforeworkcanconnue.Theexplanaonwillappearinbothpaents’cyclehistoriesandwillbevisibleintheRIWitness™Managersoware.:Ifthemismatchoccurredwhilenooperatorwasloggedin,anexplanaonforthemismatchisassociatedwiththeoperatorexplainingbutnotthemismatchitself.
Secon 5RI Witness™ Basic Operaon5Ateachworkarea,alocaonnamecanbesetthroughtheSystemInformaonwindowthatuniquelyidenesthatworkarea.InRIWitness™Manageralistofalltheconguredlocaonscanbeviewed.Whenatagisplacedintoaworkarea,thelocaonwillbestoredwiththetagdetails;thenwhenthetagsassociatedwithapaentaredisplayed,theirlastknownlocaonwillalsobedisplayed.SeeFigure5-21.Thenameofeachworkareacanbecongurediniallybyanyuser.Anadministratorcanmodifyanylocaonname.AlistoflocaonscanbeseeninRIWitness™Managerfromwheretheycanbedisabledandenabled.Disablingalocaonwillcausetherelatedworkarea’snametoberesettoUnknownsoitcanthenberenamedbyauser.Anydisabledlocaoncanbere-enabled.Thiswillsetitsnamebacktothe previous value.To name a work area:1. Whentheworkareaisstartediniallyyouwillberequiredtogivetheworkarealocaonaname.Anyusercaniniallysetthenameofaworkarea.Aredcrossiconwillappearaswellasthe  icon as a warning triangle. 2. Click the toopensysteminformaon.3. Unlockthesengs.4. Click .5. Enteralocaondescripon/name.6. Click the Icon .Whenawitnesspointiscompleted,thelocaonatwhichittookplacewillberecorded.PCs connected to the RI database must have unique computer names, if connected to a domain this will be set, otherwise please check.Figure 5-21 Tags tab
Secon 5RI Witness™ Basic Operaon355Fortrainingandtesng,anyRIWitness™workareamaybesettoTrainingMode.1. Log in as an admin operator.2. Open .3. Select .4. Thenckthebox.In Training Mode, the normal work area screen is replaced with an orange background. See Figure 5-22.OnloggingoutofTrainingMode,thetrainingworkareaeventswillautomacallybedeleted.Figure 5-22 Training modeFigure 5-23 The RI Witness™ Manager general sengs pageTraining work area events are stored in a demonstraon database which may be cleared using RIWitness™ Manager. An admin operator may click  on the  page of RI Witness™ Manager. Figure 5-23.
Secon 536RI Witness™ Basic Operaon5Designated operators may use the buon,seeFigure5-24,toassignanytagtype(spermpot, inseminated dish, sperm wash tube, etc) to an unassigned tag at any point.:AdminAssignshouldonlybeusedunderexceponalcircumstancesandbytrainedpersonnelonly.WithAdminAssignyoucanalsochangethetypeofdishalreadyassignedtoapaent.Figure 5-24 Admin assignWhen a single tag is placed in the work area the buonisenabled,seeFigure5-25.Pressingitwilldisplayaconrmaonscreenwithagreenckandredcross.The will cancel the discardandclosetheconrmaonwindow,whilstthegreenck will mark the tag as discarded - changinghowitisdisplayedintheworkareaandprevennganywitnesspointstakingplacewhilstitisin the work area.ThereisalsotheoponfromwithinRIWitness™ManagertoenableDiscardstorequireaWitness.Onceenabled,awitnesswillbepromptedtologinonthescreenfollowingtheoperatorselecngthebuon,aerasuccessfullogintheconrmaonwindowwillbedisplayedasusual.Figure 5-25 Discard
Secon 5RI Witness™ Basic Operaon375Thecyclesummaryisdisplayedintheworkareaatallmes;itisadatasheetthathasbeenselectedtobeshownfromtheRIWitness™Manager.Ifapaentisnotselected,orthereisnocyclesummaryforaselectedpaent,thecyclesummarydisplaywillsay.Iftheselectedpaentcontains a cycle summary sheet, this sheet will be displayed in the  area.Onthepaentselectwindow,youcanselectthedailylistwhichshowinglistofpaentsfortoday.Thedateisdisplayedinthetopleofthewindow.Tochooseanotherdateandviewitsrelatedprocedures,clickontheleandrightarrowsatthetopofthewindow.(Thelearrowmovesthedatebackonedayandtherightarrowadvancesthedatebyoneday).Toselectapaent,clickonthepaentIDnumber.Foradminstaonswithadminreaders,theWorkAreaapplicaoncanbewindowedtoalloweasyaccesstothetagreadingfunconality.1. OpentheRIWitness™WorkAreaapplicaon.2. Click 3. Unlockthesengs.4. Click  / .5. Tick .6. Click  and .7. Ifyouhavemulplescreenssetup,theworkareawillnowreverttoonesingleworkareascreen.Figure 5-26 Conrm discardFigure 5-27 Discard in the work area
Secon 538RI Witness™ Basic Operaon5Aerunlockingthesysteminformaonscreen,navigatetotheLanguageandKeyboardsengs.Fromthere the language on-screen and keyboard style can be changed using a drop-down menu. If the selected item in either drop down menu is changed, then clicking the  will save these changes and modify the language and keyboard.1. Click 2. Unlockthesengs.3. Select your required language from the drop down list.Inthe workareatheSystem Informaon Panelcontainsa  buonwhich launches the  dialog.Withinthekeyboardshortcutconguraondialog,eachavailablecommandislistedfollowedbyaninputboxandclearbuon(withacross).Toassignakeyboardshortcuttoacommand,clickinthecorrespondingtext/inputboxandthenholddown the keys you want to assign to this command. The input boxwill change from “PleaseEnterShortcutKey”toalistofthecurrentlyhelddownkeyboardkeysseparatedby+s.Oncethekeyboardkeysarereleasedthosekeysaresavedtotheinputbox.Pressing the buonwillremovethekeyboardshortcutforthatcommand.In order to save these changes you must press the buonattheboomofthedialog.Intheworkarea,oncetheshortcutsaresetup,simplypresstheshortcutkeystoacvatethecommand.Figure 5-29 RI Witness™ Manager Language SengFigure 5-28 Language selecon4. Select your required keyboard style from the dropdown list.5. Click the  to save and close the window.The language used can also be changed from RI Witness™ Manager1. Click 2. ChooseyourrequiredlanguagefromthedropdownlistintheLanguagesecon
Secon 5RI Witness™ Basic Operaon395List of commands:• Fire / Enter Laser Mode. ◦Enters laser control mode. ◦ Ifalreadyinlasercontrolmodethiscommandresthelaser.• Preset Laser Down.• Preset Laser Up.• Start / Stop Recording.• Take Picture.• Zoom In / Zoom Out. ◦Zooms out fully if already zoomed in. ◦If zoomed out zooms into pre-set zoom value at centre.• Zoom In. ◦Zooms into centre one step.• Zoom Out. ◦Zooms out from centre one step.• Freeze Frame.  Shortcut keys should be limited to the funcon keys to avoid conicts with typing orbarcodes.1. Log in as an Admin user.2. Click 3. Click 4. Type the name of the work area using the on screen keyboard.5. Click the Icon. Figure 5-30 Figure 5-31 1. Log in as an Admin user.2. Click .3. Click . 4. Click 5. Choosethelocaonrequiredfromthelistorclicktosetanotherlocaonname.6. Click the Icon to accept the change.
Secon 5RI Witness™ Basic Operaon51. Open RI Witness™ Manager.2. Click .3. Click ,youwillnowseeallnamedworkarealocaons.4. Highlight the work area and click .5. DisabledaccountscanbeviewedbyckingTohelpavoidmismatchesbeingrecorded–“CardLeinReader”.1. Open RI Witness™ manager.2. Click .3. Click .4. UnderworkareasengsckFigure 5-32 Figure 5-33
Secon 5RI Witness™ Basic Operaon5Figure 5-34
Secon 6RI Witness™ Manager6RI Witness™ Manager can be used on PCs or tablets outside of the RI Witness™ work areas, predominately foradministraonpurposes.1. Double click the  desktop icon.2. Choose your assigned operator name from the list.3. Enter your 4 digit PIN.4. Click Figure 6-1 Logging inFigure 6-2 License warningRI Witness™, Traceability, Data Capture, Cryo and Imaging must be licensed.LicensinginformaonisstoredinthesharedRIWitness™database.On each subsequent use of RI Witness™ Manager, a license check is made and a similar license warning windowwillinformtheoperatorifanylicensesareclosetoexpiraon.Atanyme,theRILicenseManagermayalsobeinvokedasaseparateapplicaonbyclickingand then RI License Manager.
Secon 6RI Witness™ Manager6Figure 6-3 RI License ManagerIfyourlicenseisclosetoexpiraon,youcanquicklyrenewyourlicense.1. Click in the RI Witness™ Manager license warning window. A license request form will open. See Figure 6-4.2. Complete the details on the form.3. IfyouhaveaRegistraonCodeissuedbyRI,enteritattheboomoftheform.4. Click aercomplengalltheelds.5. If internet access is available, a response will be returned by the RI Licensing Server. See Figure 6-5.Figure 6-4 The license request form
Secon 6RI Witness™ Manager66. Ifinternetaccessisnotavailable,pleasefollowthedisplayedinstruconstosavealicenserequestle.TheleshouldthenbeemailedtoRIatacvate@research-instruments.com.7. OnceRIhasreceivedyourrequest,aLicenseDatalewillbeemailedbyreturn.Figure 6-5 A response from the RI license serverFigure 6-6 The license request email8. TheLicenseDataleshouldbemadeavailabletotheRIWitness™ManagerPCwherelicensingis performed.9. EachmeRIWitness™Managerisopenedfollowinglicenserequest,LicenseManagerwindowwill be displayed. See Figure 6-7.10. OnreceiptoftheLicenseDatale,clickin the license manager dialoguebox.Browsetotheemailedlicensedataleandselect.TheLicenseManagerwillnowbe updated with the new license details.
Secon 6RI Witness™ Manager6Figure 6-7 The License Manager windowRI Witness™ Manager must connect to the shared RI Witness™ database. 1. AtRIWitness™Managerlogin,adatabaseconneconswindowwillbeshownifaconneconcannot be made. See Figure 6-8. Figure 6-8 The RI Witness™ database connecon is unavailable
Secon 6RI Witness™ Manager62. Click the pagebuontoshowthedatabaseconneconsengswindowandentertheappropriateservername,databasenameandauthencaondetails.SeeFigure6-9.See the IT Requirements Manual for more details on database management.Aerlogin,aninialscreenshowsanumberofmainmenubuons.Themainmenubuonspresentedwill depend on operator group membership (administrator, normal) and the mixture of installedproducts (RI Witness™, Traceability, Data Capture, Imaging and Cryo).When rst logging into RI Witness™ Manager the Cycles Overview Board is displayed. Clicking thebuononthelehandtoolbarwillreturntheusertotheHomescreen.ThebuonontheHomescreencausesthedisplaytorefreshbyre-queryingthedatabasetondtheacvetreatmentcycles.1. Toseethecycledetailsofapaent,doubleclickontherowwiththepaent’sdetails.A page will open.Figure 6-9 Database connecon sengsFigure 6-10 Home screen
Secon 6RI Witness™ Manager6Eachdayofacycleoraneventcanbegivenadierentcolourcodetoaideasyandquickidencaonof the cycle status. This can also assist the ordering of procedures.TocongurethecolourcodingwithintheHomescreen,followthestepsbelow.ExactspellingsofCycleTypes and Events used in the Witness Point Diagram must be used. 1. Click 2. Click 3. Firstlyyouwillneedtosetup“States”.Thesecanbedaysoreventsandarecolourcoded. YoucouldsimplydoDay1,2,3,4,5tostartwith.4. Click +withintheStateseld.5. Type the name of the State, eg Embryo Freeze, Day 0, Day 1, etc.6. Onclickingthebackgroundorforeground(TextColour)box,acolourpickerwillappear.Selectthe desired colours.7. Press 8. Nextaddacycletype.Clickthe + buonwithintheCycletypeseld.ACycleTypeboxwillappear. Type in the cycle type carefully, ensuring there are no spelling mistakes. The cycle type willneedtomatchwhatyouhavealreadysetwithintheTreatmentCycleTypeseldswithinthedatabase(See“CycleTypes”onpage70.)AnexampleofacycletypecouldbeIVF,ICSI/IVF,FET, ICSI, etc.Figure 6-11 Home screen9. Click .10. Select the  drop down arrow you would like to colour code. 11. Click or12. Assign a day number, ie 3 for Day 3. 13. WithintheMessageeld,typetheDaydescripon,ieDay3.14. IntheStatedropdowneld,choseoneoftheStatesthathasbeensetup.15. 16. Repeat for each Cycle Type.
Secon 6RI Witness™ Manager6Click the buontoshowthewindowifnopaenthasbeenselectedortoviewdetailsofaselectedpaent.1. TypetherstfewcharactersofanameorpaentIDtobrowseaseleconofmatchingpaents.2. Highlighttherequiredpaentandclickordoubleclicktherequiredpaenttoviewtheirdetails.3. Treatmentcycles,witnesspointsandtagsfortheselectedpaentcanalsobeviewedbyselecngthetabsinthisscreen.4. ClinicswithCryoenabled,canprintbarcodesfortheselectedpaentbyclicking. Barcodes printed from here will be recognised by the RI Witness™ system as belonging totheselectedpaent.5. Click tocreateadhesivesheetswiththeselectedpaentdetails.Thesecanbeaachedtoplascwareforthispaent’ssamples.Forfurtherdetailsregardinglabelprinngsee“PrinngPaentLabels”onpage54.1. Click thebuontoshowthewindow.2. Click on buon.3. Enterdetailsintoappropriateelds–alleldswhichhavebeeneditedarehighlightedinpink.4. Save all changes. FromthePaentspagethereisalsoanopontoaddorchooseanewpaent. A paent cannot be assigned to a partner unl the partner has also been entered into the RIWitness™Managersowareseparately–See“AssigningPartners”onpage50. Label sheets and barcodes can be printed from this page. Figure 6-12 Choose paent screen
Secon 6RI Witness™ Manager6Figure 6-13 New paent detailsOpenPaentdetailsasoutlinedabove.1. Click the buon to change to the editable window. 2. Click the buontosavechanges.1. FromthePaentspage,selectthe tab to view all previous cycles undertaken forapaentorcouple.2. Select the  tab to view all witness points registered in an open cycle.3. Select the tabtoseealllocaonsthattagsassignedtothepaenthavebeenseenbyRIWitness™.RefertoFrequentlyAskedQuesonsifnecessary.Figure 6-14 Looking up paent histories
Secon 650RI Witness™ Manager6Figure 6-16 Assign Partners1. Createanewpaententry,orchooseexisngpaent,leavingthepartnereldblank.2. Createanewpaentforpartner.3. Tolinkthe2paents,selectthebuontoshowtheChoosePaentwindow.4. LocatepartnerbytypingtherstfewcharactersofanameorpaentIDinthesearchwindowtobrowseaseleconofmatchingpaents.5. Clickonthepaentnameandthenconrmseleconbyclicking. See Figure 6-16. InthePaentdetailswindowselectrelevantdonoropons.Can Receive Untagged DonationsCan Receive Tagged DonationsCan DonateFigure 6-15 Assign Donor StatusTheavailability of the donatecheckboxes will dependonthegenderof the paent,ie only femalepaentscanreceivedonaons.
Secon 6RI Witness™ Manager516Acyclerelatestoaspeciccourseoftreatment(IVF,ICSI,IUI,etc).Only“administrator”operatorsmaycreatecycletypes.1. Click the mainmenubuonandthenclick to view all treatment cycle types. See Figure 6-17.2. Click  to add each cycle type. 3. Use a right click to  a cycle type.Traceability operators may specify the range of materials that are used in a cycle type. Data Capture operators may specify the data sheets that are used in a cycle type. Figure 6-17 The Treatment Cycle Types Window1. Click the mainmenubuonandselectapaentbyenteringanameorpaentID.2. Click the tabtoshowthelistoftreatmentcyclesforthispaent.Foranewpaentthislistwillbeempty.3. On the tabofthepaentscreenclick to create a new cycle.Thenewcycleisshownwithastatusof“InProgress”andacycletypeof“Unknown”.SeeFigure 6-18.4. Traceability operators may click the  tab to view a list of material batches related to this cycle. 5. All operators may click the  tab to view a list of witness points performed during this cycle.Select a cycle type from the pulldown list.
Secon 652RI Witness™ Manager6Figure 6-18 Starng a new cycle.1. Click fromthePaentswindow.2. Select a Cycle Type from the pulldown on the window.IfEggCollecon/EmbryoTransferdatesareenteredonthe tab of a treatment cycle then these events will be available from the work area touchbuon.See“PerformingAProcedureinRIWitness™”onpage28formoredetailsofpaentseleconusing.Anewlycreatedcyclewillbeassignedthestatus“InProgress”.Whenthepaenttreatmenthasbeencompleted click tochangethestatusto“Completed”.An administrator operator may click the  buonofa“Completed”cycletoreturnthestatusto“InProgress”.
Secon 6RI Witness™ Manager536LaserprintableadhesivepaentidentylabelsareavailablefromRI.Thesheetprovidesarangeoflabelsizeswithaseleconofnormalandreverseprinng.SeeFigure6-19. Figure 6-19 RI laser printable label sheet.Reverse printed labels are viewable through the base of containers.Label categories such as “Petri Dish” and “Test Tube” are for guidance only. Please select a labelappropriatetoyourplascware.“Testtube”labelsmaybeusedtoholdarectangularRFIDtaginposionalongthelengthofatube.Thereverseprinted“4welldish”labelandthesquareRFIDtagaretherecommendedchoicefora4well dish. See Figure 6-20.RILabelsareanoponalcomponentofRIWitness™.Figure 6-20 Posioning of paent identy labels
Secon 6RI Witness™ Manager61. FromthepaentdetailswindowclickSee Figure 6-21.2. Load a blank label sheet into a laser printer. For repeatability of alignment use a manual feed path and take care when placing each sheet.3. Setall“SheetAlignment”valuestozeroandclick.4. Checktheprintedsheet.Measuretheosetsrequiredtocorrectlyposiontheprinteddetailswithinalabelboundary.Payparcularaenontovercalposioningwithinthesmallestlabel.See Figure 6-22.Figure 6-21 The label prinng managerTheexampleshowninFigure6-22showsdetailsofanalignmentcorreconwithintheboundaryofthesmallest label.5. Therstprintshowsincorrectalignment.6. VercalalignmentiscorrectedbyanegaveYoset.7. HorizontalalignmentiscorrectedbyaposiveXoset.
Secon 6RI Witness™ Manager556Figure 6-22 Label text alignmentUsetheLabelLayoutbuonstoselectanappropriatestyle.Style1isopmisedforpopularplascware,Style2maximisestheuseofavailablespaceandStyle3isamixtureofStyles1and2.Arotaonalignmentcorreconisrarelyrequired. Check  and select a row number to print a single row of the smallest labels only. This may be useful if reusing a sheet during the alignment process.Click  to display a list of all enabled operators. See Figure 6-23.Figure 6-23 Operator management
Secon 656RI Witness™ Manager61. Click to specify details for a new operator. See Figure 6-24. 2. Select an operator and click to change operator details.An operator must have a Username, Full Name and Anonymous ID. The Anonymous ID is used for printed reports.Groupmembershipisspeciedas“normal”or“administrator”.Some RI Witness™ features are only available to an “administrator” operator. Administrator onlyfeatures include the use of admin assign and making changes to the witness point diagram.Figure 6-24 Operator DetailsOnly enabled operators may log in. Click  to remove an operator from the enabled list. Check “ShowDisabledAccounts”toviewalloperators.The operator PIN must be a sequence of 4 numbers.Click the mainmenubuonthenclick to specify the Name of Clinic that will appear in all printed reports. SeeFigure 6-25.
Secon 6RI Witness™ Manager576Figure 6-25 The heading of a printed report will show “My Clinic Name” as specied by “Name of Clinic” on the general sengs page.Userscanaddextracommentstofurtherexplainanymismatchesinthehistory1. Open RI Witness™ Manager. 2. Click the buon.3. From double click the cycle required to view. This can be seen by clicking paentsalso.4. Click the  tab. 5. Double click the  witness point.6. Click the  icon.7. Enteraddionalcomments.8. Click Figure 6-26 Report sengs
Secon 658RI Witness™ Manager6ThewitnesspointsthatarepresentedtoallworkareaoperatorsaredenedinRIWitness™Managerby a witness point diagram.To view the witness point diagram click the mainmenubuonandthenclick. See Figure 6-27 and Figure 6-28.Figure 6-27 The sengs screen Click “Witnessing” to view the witness point diagramTo add a new witness point, click  and then click anywhere in the diagram. Enter a nameforthewitnesspoint,eg“IVFInseminaon”UsetheWitnessPointInfopaneltospecifytheaributesofawitnesspoint.Witnesspointaributes,egreassignan“eggculturedish”tagtobecomean“inseminateddish”tag,aredescribedbelow.Tagnamessuchas“inseminateddish”mustbedenedbeforetheymaybereferencedbyawitnesspoint.See“TagTypes”onpage63.Figure 6-28 The witness point diagram.
Secon 6RI Witness™ Manager596Figure 6-29 Detailed informaon for the “IVF Inseminaon” witness pointThelinks(arrows)betweenwitnesspointboxesrepresenttags(dishes,testtubes,etc)thataretherequiredinputsandproducedoutputsoftheproceduredenedbythatwitnesspoint.To create a link, click  and then drag between two witness points.Awitnesspointmayrepresentthereassignment(transformaon)ofonetagtypeintoanother,egan“EggCultureDish”becomesan“InseminatedDish”asthespermisintroduced.A reassignment is illustrated in Figure 6-29 where the checkboxischeckedtospecifythattheselectedtagtype“EggCultureDish”willbereassignedasan“InseminatedDish”whenthiswitnesspointisexercisedbytheworkareaoperator.Witness points may require that unassigned (empty, unused) tags be introduced into the work area.The“IVFInseminaon”WitnessPointdoesnotrequireanunassignedtagasthe“Unassignedtagswillbeassignedto:”pulldowninFigure6-29shows“none”.Whereawitnesspointrequirestheintroduconofanunassignedtag,usethe“Unassignedtagswillbeassignedto:”pulldowntoselectthetargettagtype.Paent ID (female)IVF Inseminaon Egg Culture DishInseminated DishFigure 6-30 A reassigned tag is represented by a curved line between the two tag namesAnunassignedtagisrepresentedbyastraightlinebetweenaquesonmarkandthetargettagname. A witness point may specify both a reassignment and the use of an unassigned tag.
Secon 660RI Witness™ Manager6Incoming links to a witness point specify the tags that must be present before the procedure represented bythewitnesspointcanbeperformed.Ifanunassignedtagisspeciedthenthatcontainermustalsobe present before the procedure can be performed.Awitnesspointwithmulpleinputlinksofthesametagtypeeg“dishX”,representsanORoperaon,ie the required container may be sourced from the procedure represented by witness point A OR B.A BCDish X Dish X Figure 6-31 Dish X must be available to witness point C Witness point A OR B may be the sourceAwitnesspointwithmulpleinputlinksofdieringtagtypesrepresentsanANDoperaon,iethistagtype AND that tag type are required to perform the procedure represented by this witness point. See Figure 6-32.A BIVF InseminaonSperm Wash Tube Egg Culture DishInseminated DishFigure 6-32 The “IVF Inseminaon” witness point represents a procedure that requires a “Sperm Wash Tube” AND a “Egg Culture Dish”Entry(inial,starng)witnesspointshavenoincominglinks.Anentrywitnesspointwillalwaysrequireanunassignedtagwhichwillbecomeassignedoncompleon.SeeFigure6-33.Figure 6-33 “Assign Paent ID (female)” is an entry witness point as it has no input links An unassigned tag (here an unassigned ID card ) is requiredAssign Egg WashEgg Wash DishPaent ID (female)Assign Paent ID (female)? → Paent ID (female)Checking the box,seeFigure6-29,changesthecompleonsequenceforawitnesspoint. A second operator must log in to approve the procedure. Entry witness points are generally double witness points.
Secon 6RI Witness™ Manager616Amismatchalarmwillbegeneratediftagsassignedtopaentswhoarenotspeciedaspartnersareintroduced to the work area.Theexcepontothisruleisprovidedbydonorwitnesspoints.Checkingtheboxwillcreateadonor witness point which is given a diagonal colouring. See Figure 6-34.Assign Egg WashEgg Wash DishEgg PrepDonate EggsFigure 6-34 A known donor witness pointAnincominglinktoadonorwitnesspointspeciesataggeddonaonandcoversproceduressuchasegg/embryodonaon/sharingaswellassurrogacy.Whenthewitnesspointisselectedtheoperatorchoosesthedonaonrecipient.Therecipientidentyisassignedtothetagcontainingthedonatedmaterial.Theworkareanowcontainstagswithamixtureofdonorandrecipientidenes.Thisdoesnottriggeramismatchbecausethetagshavethedonorwitness point in common.TaggedDonaonsrequirethatthedonorandrecipientbespeciedassuchviathe and checkboxesavailableinthepaentdetails.SeeFigure6-35.Can Receive Untagged DonationsCan Receive Tagged DonationsCan DonateFirst NameLast NameDate of BirthWendyRogers27 October 1967Partner 36734M Mr John RodgersFigure 6-35The   checkbox speciestheabilitytomakeataggeddonaon.The     checkbox must be checked for therecipient.The donor witness point ensures that donated tagged material is only available to paents that  have  the    boxcheckedintheirpaentdetails.
Secon 662RI Witness™ Manager6Spermdonaonsareusuallyuntagged.An donor point will be an entry witness point for a female recipient of donated sperm. The donated sperm will be transferred to an unassigned tag that is then assigned to the female recipient when the donor witness point is selected.Thedonorwitnesspointensuresthatuntaggedspermisonlyavailabletopaentsthathavetheboxcheckedintheirpaentdetails.Aquesonmaybepresentedtoaworkareaoperator.Thequesonandresponsewillberecordedinthe history log.Tosetaquesonfortheselectedwitnesspoint:1. Click the buon.2. Chooseaquesontypeandenterthequesontext.SeeFigure6-36.3. The diagram will show an appendedtothewitnesspointnameifaquesonhas been added. 4. Click the toeditthequesondetails.Figure 6-36 Adding a witness point queson that requires a numerical answer Note the exclamaon aer the witness point namePaent ID (female)IVF Inseminaon Egg Culture DishInseminated Dish!!!
Secon 6RI Witness™ Manager636Click   to specify the name and icon for each RFID tagged item used in the witness point diagram. See Figure 6-37Figure 6-37 Tag typesFrom the witness point diagram page click  to specify the order in which points will be displayed to the work area operator. Click  or  to change the order. See Figure 6-38.Figure 6-38 Seng the witness point order.Workareaevents,egwitnesspointseleconandtagmismatches,areloggedintheshareddatabase.Thewitnesspointlog maybeexplored in detailor viewed as a summary. A printedreport maybegenerated from any view of the witness point log.Click the mainmenubuonthenclick or  to view the witness point log. See Figure 6-39.
Secon 6RI Witness™ Manager6Figure 6-39 The witness points screen Click stascs or explore to view the witness point logTheWitnessPointExplorershowseventsfromthewitnesspointlog.Theviewmaybelteredbydate,operator,paentandtagtype.Shortcutlinksareprovidedforcommonlters,eg“Monday”,“Adminassignsthisweek”.SeeFigure6-40.Figure 6-40 Exploring the witness point logThestascsviewsummarisesthewitnesspointlogbypresenngsignicanttotals,eg“Tagsused”,“Paentsseen”,etc.Theviewmaybelteredbydate.SeeFigure6-41.Figure 6-41 Stascs from the witness point log
Secon 7Cryo Witnessing657Many embryology labs wish to extend witnessing and reporng to include samples stored in cryostorage.TheCryofeatureisabarcodelabellingsoluonforcryostraws.Clinicshavetheoponofscanningbarcodesthattheyhavealreadybeenassignedtosamples,andRIbarcodesthathavebeenprintedfromRIWitness™Managerandareassignedtoaparcularpaent.IfacliniconlywishestobeabletoscanabarcodeprintedfromtheRIWitness™Managerapplicaon1. Open .2. Click 3. Click 4. TickthecheckboxundertheBarcodesecon.Figure 7-1 Only use RI barcodes1. Open 2. Fromthepaentdetailswindowclick. See Figure 7-2. Figure 7-2 RI Witness™ Manager
Secon 766Cryo Witnessing7Figure 7-3 Barcode prinng window3. To print barcodes make sure that a correct label printer is plugged into the computer with the correct label stock loaded into it.4. Select • The required number of barcodes• Thestarngnumber• The freeze date of the barcodes. 5. A preview of the barcodes that will be printed is shown in the . The last number onthesecondlineisthesamplenumberforthispaent.Thiscanbeeditedallowingreprinngofaparcularbarcode,forexampleiftheinialprinngwasmisaligned.6. Click . 7. If you are using small style labels you may need to print a blank row to gain access to the printed labels. To print a blank row of labels, click . 8. Tochecktheprintedlabels,measuretheYOsetbetweenthetopofthebarcodeandthetopofthenextlabelandsettheprintheadposionontheprintertothisvalue.Printheadpos.Y+7.2 mm12Figure 7-4 Aligning the label print stock 1. The rst print shows incorrect alignment. 2. Alignment is corrected by seng the printhead Y posion
Secon 7Cryo Witnessing677Intheworkareaapaentcanbechosenbyscanninginasamplebarcodethathaspreviouslybeenassignedtothispaent.When compleng a witness point that includes a barcoding step, the barcode, either a brand newbarcodeorabarcodepreviouslyassignedtothecurrentpaent,mustbescannedpriortotheWitnessPointConrmaonscreen.SeeFigure7-5.Figure 7-5 Scanning a barcodeIfthebarcodehasnotbeenseenbythesystembeforeitwillbeassignedtothepaentaerthewitnesspointisconrmed.Ifthebarcodehasalreadybeenscannedandassignedtoadierentpaent,themismatchalarmisacvatedandthedieringpaentisshownonthemismatchscreen.Clickingonthesmall nexttothedisplayedmismatchedpaentwillallowthemismatchscreentobeclosedandareasonaached.SeeFigure7-6.Figure 7-6 Barcode mismatch
Secon 868RI Witness™ Manager for Traceability8Materialtypes,egdishes,pipeesandmediamustbedenedforusewithinTraceability.Abarcodereadermaybeusedtoidenfybatchlotnumbers,expirydates,etcorthesedetailsmaybeenteredmanually.Batchesareopenedorclosedtoreecttheiravailabilitywithinthelab.Click the mainmenubuontoviewthematerialsrelatedfeaturesofTraceability.SeeFigure8-1.Figure 8-1 Materials managementMaterialtypesmustbeconguredbeforethedetailsofadeliveredbatchmayberecorded.1. Click the mainmenubuon.2. Then click  to view a list of all material types.3. Use  and  to specify the top level of your materials hierarchy. See Figure 8-2.4. Right click a group and select toextendthehierarchy.5. Right click a group and select  to add a new material type to that group. See Figure 8-3.Figure 8-2 Viewing all material types
Secon 8RI Witness™ Manager for Traceability698Figure 8-3 Right click to add a sub groupDouble click a material type, or right click and select  to view the material type details. See Figure 8-4.Figure 8-4 Material type detailsSomematerialsaremarkedwithaGS1barcode.AGS1barcodemaycontainvariouscombinaonsofexpirydate,lotnumberandauniqueproductidenerknownasaGTIN.Hereweareonlyinterestedinaproductidener.1. If you have a barcode, click  and scan the barcode. 2. Manually enter a unique product part number if no GTIN is available.
Secon 870RI Witness™ Manager for Traceability8GS1isaglobalbarcodestandardthatmaycontainaUniqueProductIdener(GTIN)andmayalsocontainlotnumberandexpirydateinformaon.SomeproductshaveabarcodethatdoesnotadheretotheGS1standard.SomeGS1barcodesdonotcontainalltheinformaonhighlightedabove,egaboxofbolesmayhaveabarcodethatcontainsGTIN,lotandexpiryinformaonbutanindividualbolebarcodemayonlyhaveLotandExpiryinformaonbutnoGTIN.Traceability can interpret GS1 barcodes.A Traceability buon must be clicked to interpret a GS1 barcode, eg click   or   or .Treatmentcycletypesmustbeconguredandlinkedwithmaterialtypes.1. Click .2. Then .3. Then the  tab to view the materials linked to a cycle type.4. Tick the materials within the list that are to be associated with the treatment cycle type.5. Press .Aspreviouslydescribedin“Secon6-RIWitness™Manager”onlyadministratoroperatorsmaycreatecycletypes. Traceabilityadminoperatorsmayaddionallyconguretherangeofmaterialsthatareused when performing a treatment cycle type.Anewlycreatedcycletypewillnotbeassociatedwithanymaterials.Usethematerialscheckboxestospecify the range of materials that will be used for the selected cycle type. See Figure 8-5.A new batch of a GTIN barcoded material may be scanned directly into Traceability. 1. Click themainmenubuonandthenclickthebuon.2. Youwillbepromptedtousethebarcodescannertoreadthebarcode.Scanthebarcode.3. As the materials type is already logged in the system, it will recognise the GTIN barcode and add thenecessarydetailsofthatbatchofmaterialstothesystem,eglot,expirydate,etcwithintheBatch Details page.Materials that are not GTIN barcoded are selected by navigang to the appropriate “material typedetails”screen.1. Click the mainmenubuonthenclick to show a list of all materials.2. Double click the material that is being processed to show the Batch Details page for that material. See Figure 8-7.3. Alistofexisngbatchesofthismaterialisshown.Click to open the batch detailswindowforanewlycreatedbatchandllinthebatchdetails.SeeFigure8-6.4. Click  to create a new batch if a GS1 barcode is available.
Secon 8RI Witness™ Manager for Traceability718Figure 8-5 Material type details checkboxes specify the range of materials associated with each cycle typeFigure 8-6 Creang a new batch by scanning a barcode
Secon 872RI Witness™ Manager for Traceability8Normalbatchoperaonsarestatuschanges,eg• : The batch of materials will shortly be opened and used•  The batch of materials has been opened and is in use within the lab• Thebatchofmaterialshasbeenclosed,hasallbeenusedup,hasexpired,cannolongerbe used. Batchesarenotrounelydeleted.If a batch is created in error then an admin operator may delete it.1. Click the mainmenubuon.2. Click .3. Double click the material type to be deleted.4. The list of batches will be shown. Right click the appropriate batch and select .Anewlycreatedbatchwillbeassignedastatusof“Pending”.Click when the batch is made availableforuse.Thedetailsdisplayedforanopenbatchwillincludeallpaenttreatmentcyclesthatare in progress. See Figure 8-8.When a batch is no longer in use, click tochangethebatchstatusfrom“Open”to“Closed”.If a batch is opened in error, click the buon toreturnthebatchstatusto“Pending”.If a batch is closed in error, click the buon toreturnthebatchstatusto“Open”.Figure 8-7 Lisng all batches of a specic material type Click “Add Batch” to manually create a batch
Secon 8RI Witness™ Manager for Traceability738Figure 8-8 Paent treatment cycles related to a batch of materialsWhenbatchesarecreatedanexpirydateisrecorded.Ifanycurrently“Open”or“Pending”batchesareexpiredorwithin7daysofexpirythenanexpirybuonwillbeavailableonthemainMaterialsscreen. See Figure 8-9.Doubleclickanexpiredbatchtoshowthebatchdetailsscreenfromwhichthebatchmaybeclosed.Now remove the batch from use.Theexpiryperiodcanbesettoyourrequiredmeperiod,ieextendedto10daysorshortenedto5days. 1. Click in the main menu.2. Click .3. WithintheMaterialsseconamendthebatchexpirywarningeld.Figure 8-9 Expired and expiring batches
Secon 8RI Witness™ Manager for Traceability8From the main Materials screen click toviewallbatches.Alteredlistofallbatchesisshown,expiredbatchesarehighlightedinred.Thislistmaybelteredbydateandbybatchstatus.SeeFigure8-10.Figure 8-10 A ltered view of all batchesSee“Secon5-RIWitness™Basicoperaon”fordetailsofcreangandmanagingatreatmentcycle.When viewing the details of a treatment cycle Traceability operators may click the Materials tab to show material batches related to this cycle. See Figure 8-11.Batchescanbeexcludedfromcyclesbyrightclickingonthebatchandchoosing on the contextmenu.Excludedbatchescanbeviewedorhiddenbyclickingthebuonabovethebatcheslist.Excludedbatcheswillbehighlightedblue.Anexcludedbatchcanbeincludedagainbyrightclickingonanexcludedbatchandfromthecontextmenuleclicking. The batch will now be visible in the list of batches for the cycle.Figure 8-11 Material batches related to this cycle
Secon 9Data Capture759Hand wrien notes are oen taken whilst performing laboratory procedures. For example embryoscoresandspermvolumesmightbewriendownonadatasheetandlatermanuallytranscribedintoaclinicferlitydatabase.The features of Data Capture described in this chapter allow data sheets to be designed in RI Witness™ Manager and data entry to be performed in the laboratory using the work area touch screen. Data entry may also be performed in RI Witness™ Manager.1. Click  2. Then click toenterthesetupscreenfordatacollecon.Sheetdesignisperformed using this screen.The Collected Data Setup screen, Figure 9-1, is divided into 4 regions. Note that region 4 shows the properesofanentryselectedfromregions1,2or3.Figure 9-1 Collected data setup13 2  Region 1 shows a list of all the sheet types that have been created. Click  to add a new sheet, setthenameofthenewsheetusingtheproperesinregion4.  Parametersareeldsthatmaybeplacedonadatasheet.Forexample,aparameternamed“SpermVolume”thatrequiredanumericalvaluemightbeaddedtoan“Andrology”datasheet.Region 2 lists the parameters that have been created and may be added to the contents of a sheet.Click the buontoaddaparametertoasheetcontents.Theparameterwillmovefromregion 2 to region 3.
Secon 976Data Capture9  Region 3 shows the content layout of the selected sheet type. The contents of a sheet are parameters that have been added to the sheet from region 2. To change the layout order, highlight the parameter by clicking it. Then use the  and buonstoposionitintheorder required.Onceaparameterhasbeenaddedtoasheetcontents,addionalaributesmaybeset.Forexample,andefaultvaluecanbesetoraparametercanbemarkedasrequiredsothatanoperator must provide a value.See“ParameterAributes”onpage87formoredetails.  Theproperesshowninregion4reectthemostrecentlyselectedentryfromregions1,2or3.See Figure 9-2. Figure 9-2 Examples of parameter properes and sheet properes shown in region 4The contents of a sheet are called parameters. 1. Click intheparametersregion(2)tocreateanewparameter.Theproperesregion(4)willshow details of the newly created parameter. See Figure 9-3.2. Enteranameforthenewparameter,eg“SpermVolume”.3. TherstacontoperformonanewlycreatedparameterwillbetoselectaparametertypefromtheProperesTypedropdownmenu.Parameterproperesdependontheselectedparametertype.Forexamplethetexttypehasproperesminandmaxlengthwhereasacalculaonhastwooperandsandanoperaon.Figure9-3showstheproperesofeachparametertype.Figure9-4showstheParameterTypedropdown menu.Theproperesofanumericalparameterincludeaminimumvalue,amaximumvalueandanoutputformat.Theformatspeciesthenumberofdecimalplacestobeusedwhendisplayingnumbers.Format meansthatthevalueenteredbyanoperatorwillnotbeformaed.Figure9-5showstheFormat pull down.Theproperesof a textparameter include a minimum length and a maximumlength.Leavetheseproperes blank if length restricons are not required when the operator enters a value for thisparameter.
Secon 9Data Capture779Figure 9-3 Parameter properes depend on the selected parameter type Select from: Number, Text, Check, List, Date, Time, Date Time, Built In and CalculaonFigure 9-4 Selecng a parameter typeFigure 9-5 Selecng a numerical formatFigure 9-6 The properes of a list parameterListparametersallowtheoperatortochooseavaluefromapre-denedlist.Theproperesofalistparameter are shown in Figure 9-6.Click the buontoshowtheManageListswindow.TheexampleshowninFigure9-7highlightsthe“PolarBodyPosion”listthatcontainsthe3values,  and .The“PolarBodyPosion”listisassignedtothe“”listparameter.SeeFigure9-6.CheckparameterstakeYes/Novalues.SheetspresentedbyRIWitness™Managerwillshowcheckboxes.Aworkareatouchscreenwilloeraseleconof or .
Secon 978Data Capture9Figure 9-7 The manage lists window Figure 9-8 Seng a date me valueFigure 9-9 Adding two numbers using a calculaon parameter Figure 9-10 Selecng a built in valueDateandmevaluesareviewedandeditedusingRIWitness™Manager.Anoperatormaytypeavalue,eg 12:30 or 14/08/2010 15:35. A date picker window is also available. Figure 9-8.Calculaonsperformanoperaonontwooperands,egaddtwonumbersorjointwopiecesoftext.Theoperandsofacalculaonmaybeparametersofanytypeincludingcalculaontypes.AexamplecalculaonisshowninFigure9-9.Builtinparameterswilldisplayvaluesderivedfromtheassociatedtreatmentcycle,egtheeggcollecondate. Figure 9-10 shows the available built in values.Click the parameters buontomoveparameterstoasheetcontents(region3).Intheexampleshown in Figure 9-11, four list parameters have been added to the contents of the sheet.Figure 9-12 shows another example of sheet contents where two list parameter and four numberparameters form the contents of the Sperm Pre Prep sheet.
Secon 9Data Capture799Figure 9-11 Adding parameters to the Embryo Development sheetFigure 9-12 Adding parameters to the Sperm Pre Prep sheetA parameter may be part of the contents of many sheets. The same parameter may be editable on sheet A and sheet B and but not editable on sheet C. A parameter that appears on many sheets has asinglesharedvalue.IntheaboveexampleavaluechangeonsheetAwouldautomacallyappearchanged on sheets B and C.Thecontentsofasummarysheetmighttakeparametersfrommanyothersheets.See“CycleSummarySheets” on page 86 for more details. See “Parameter Aributes” on page 87 for more details oftheeditable/noneditableparameteraribute.
Secon 980Data Capture9Figure 9-13 Use the checkboxes to associate sheets with the selected treatment cycle typeSheets may be viewed and edited on a laboratory work area touch screen.If sheets have been associated with the treatment cycle type being performed, then a  buonwillbeshownonthetouchscreen.SeeFigure9-14.Touch the buontoseealistofavailablesheets.Touchabuontovieworeditthe sheet values. See Figure 9-15 and Figure 9-16.Use the touch screen  and arrowbuonstohighlightthesheetvaluetobeedited.Nocethatthetypeofthevaluerequiredisindicatedtotheleofthevalueentrybox,eg123fornumericalinput,ABCfortextinputandindicanguseofthearrowkeysforlistandYes/Noinput.Treatmentcycletypes,egIVF,ICSIorIUI,willhavedierentdatacolleconrequirements.Forexample,a sheet designed for IUI would not be used during an ICSI treatment. 1. Click  then  to associate sheet types with a treatment cycle type.2. TheDataCollecontablistsallavailablesheets.Checktheboxesofthosethatareassociatedwith the selected cycle type. See Figure 9-13.3. Click .Whenalistofsheetsispresentedtoanoperator,theywillbeorderedasspeciedhere.Usethe and buonstochangethatorder.During a treatment cycle an operator may view and edit the values on a sheet. Each treatment cycle will start with blank copies of the sheet types that have been associated with the treatment type being performed.
Secon 9Data Capture819Figure 9-14 Touch the “Data Sheets” buon to view or edit all sheets associated with this treatmentFigure 9-15 Touch buons for each available sheet are shown in the work areaFigure 9-16 Use the touch keyboard to edit sheet values
Secon 982Data Capture9Data values will most likely be entered at the work area touch screen but sheets may also be viewed and edited using RI Witness™ Manager.1. Selectapaent.2. Click the  tab and then double click a cycle to show the Treatment Cycle screen.3. Click the  tab to view or edit any sheet associated with this treatment shown in Figure 9-17.TheproperesofaparameterincludeasengthatdefaultstoCycle,ieatreatmentcycledoesnothavemulplevaluesforthisparameter.SeeFigure9-18.However many parameter values will relate to an individual egg. Change the sengfrom“Cycle”to“Egg”forsuchparameters.Eggrelatedparametersaredisplayedtotheoperatorasatablecontaining one row for each egg. See Figure 9-19 and Figure 9-20.On the work area touch screen, touch a row of the egg table to edit the values for that egg. See Figure 9-21. The circulararrowbuonsatthetopof thescreenmaybeused toselect anothereggwithout returning to the table view.Figure 9-17 Eding sheet values with RI Witness™ ManagerFigure 9-18 The relates to seng defaults to cycle
Secon 9Data Capture839Figure 9-19 A RI Witness™ Manager view showing a table of egg related parameters.Figure 9-20 A RI Witness™ work area touch screen view showing a table of egg related parametersFigure 9-21 Entering values for egg number 1 at the work area touch screen
Secon 9Data Capture91. Click .2. Then to view a list of treatment types.TheDataCollecontabisusedtoassociatesheettypeswithatreatmenttypeasdescribedearlierinthischapter.TheDataCollecontabalsooersanEggCountParameterseng.Thedropdownmenuforthe Egg Count Parameter is a list of all parameters. Select one parameter that will contain a numerical value. The selected parameter will control the number of rows in any table of egg related parameters.The parameter selected to represent the egg count can be any parameter with a numerical value that relatestoacycle.InFigure9-22theparametercalled“TotalNumberofEggs”hasbeenselectedfroma list of all parameters.Asanexample,“TotalNumberofEggs”mightbeacalculaonparameterwhoseoperandsareotherparameters. See Figure 9-22.Figure 9-22 The number of eggs involved in a treatment is set using the egg count parameterFigure 9-23 This calculaon parameter has been selected as the egg count parameter in the example shown in Figure 9-22Changes to the value of the parameter set as the  will change the number of rows in any table of egg related parameters.Ifthenumberofeggsisincreased,thenthenumberofrowsinalleggtableswillautomacallyincrease.Ifthenumberofeggsisdecreased,thenthenumberofrowsinalleggtableswillnotautomacallydecrease. A decrease in the number of eggs will be indicated in RI Witness™ Manager as an . See Figure 9-24.
Secon 9Data Capture859A conict resoluon window is shown when the    buon is clicked. SeeFigure9-25.Theconictresoluonwindowallowstheoperatortomanuallyselecttheeggrecordsthatwillbedeletedtoachievetherequireddecreaseineggcount.Theconictwillberesolvedwhenthenumber of egg records equals the value indicated by the Egg Count Parameter. If it is decided that a decrease must be “undone”, then click the  buon to exit the conictresoluonwindowandincreasethevalueoftheEggCountParametertomatchthenumberofeggrecords.Figure 9-24 The Egg/Embryo Count Conict buon is shown if the value of the parameter selected as the egg count parameter is decreasedFigure 9-25 The Egg/Embryo Count Conict window
Secon 986Data Capture9The work area touch screen buonoersaccesstoallthedatasheetsthathavebeenassociated with a treatment type.Amoreselecveapproachtodatacolleconispossiblebylinkingasheettypetoawitnesspoint.SeeFigure 9-26.When a touch screen buonistouched,thelinkedsheetwillbeautomacallypresentedso that the operator may enter data related to the procedure being performed. See Figure 9-27.Figure 9-26 The selected witness point “IVF Inseminaon” is linked to the data collecon sheet “ 4 Inseminaon/ICSI”, see above at boom rightFigure 9-27 A witness point will trigger the presentaon of a linked data collecon sheetAny sheet type may be selected as the cycle summary sheet.1. Click  in the main menu bar.2. Then click .3. Use the  drop down menu to select the sheet type that will become the summary sheet for this treatment type. See Figure 9-28.
Secon 9Data Capture879Figure 9-28 Select one sheet to become the cycle summary sheetFigure 9-29 Touch the cycle summary buon to see the cycle summary sheet Touch again to return to the original displayAtypicalsummarysheetwouldshowparametervaluesfrommanyothersheets.See“SharedBetweenSheets”onpage79formoredetails.Quick access to a read only view of the cycle summary sheet is visible in the work area via the touch screen Buon.SeeFigure9-29.Parameters that have been added to a sheet contents may be given sheet specic aributes. Click then . See Figure 9-30.Click in the column and enter a value.Inialvaluesarelinkedwiththe and checkboxes.Aninialvaluesengwillbeignoredunlessacombinaonof and or  is also checked. See Figure 9-31.
Secon 988Data Capture9Thevalueofeditableparametersmaybechanged.Ifthischeckboxisnotcheckedthentheparameterwillbeshown“greyedout”andmaynotbechangedonthissheet.Anoperatormustenteravalueforarequiredparameter.Thesheetcannotbesavedunlavalueisentered. Required parameters are highlighted with an asterisk on the work area touch screen and in red when viewed in RI Witness™ Manager.The value may not be editedn/aEdingallowedbutmaybeleblank.Avaluemustbeenteredbutitmaynotbeleblank.Ifavaluealreadyexistsitwillbeoverwrienwiththeinialvalue.Theusercannotchangethevalue.n/aIfavaluealreadyexistsitwillbeoverwrienwiththeinialvalue.Theusermaychangethevalue.Ifavaluealreadyexistsitwillbeoverwrienwiththeinialvalue.TheusermaychangethevaluebutitmaynotbeleblankIfnovalueexiststhenshowtheinialvalue.The user may not change this value.n/aIfnovalueexiststhenshowtheinialvalue.The user may change this value.Ifnovalueexiststhenshowtheinialvalue.Theusermaychangethisvaluebutitmaynotbeleblank.Theinialvaluebeshown,overwringanyexisngvalue.The user may not change this value.n/aTheinialvaluebeshown,overwringanyexisngvalue.The user may change this value.Theinialvaluebeshown,overwringanyexisngvalue.Theusermaychangethisvaluebutitmaynotbeleblank.Whatdoesthiscombinaonmean?Figure 9-31 Combining parameter aributesFigure 9-30 Seng parameter aributes. Inial Value, Editable, Overwrite, Create and Required
Secon 10Imaging8910A camera will need to be connected to the PC running RI Witness™ and the drivers installed correctly toviewliveimageswithinRIWitness™.TheImagingsecuritydonglemustalsobeedtoaUSBportand a valid Imaging license issued. RIWitness™sowarewillautomacallyrecognisebothanalogueandcompabledigitalcameradevicesconnected to the PC. If there is only one camera device detected, it will show the live image for that device.Ifmulplecameradevicesareconnected,itwillusethecamerathatwaslastselectedfromthevideosourcedropdownbox.IftherearemulplecamerasaachedtothePC,thedeviceyouwanttheliveimagetobeviewedfrommay be selected. To do this, select the Camera from the dropdown list on the  panel. MulpleImagingscreenscanbeconguredforusewithImaging.1. Open 2. Unlocksengs.3. Touch .4. SetyourdesiredconguraonIftheworkareaiswindowed,thenmulpledisplayswillnotbeshown.WindowedworkareaisrecommendedforAdminPCswithreadersconnected.Toenablesimplyckthebox.5. ThecurrentoperatorandpaentwillbedisplayedonthesecondaryImagingscreen.BothoperatorsandpaentsmaybechangedbyclickingtheappropriateareaontheImagingscreen.Imagesarecapturedforthepaent’scyclecurrentlyacveintheworkarea.ImagescapturedfortheacvecyclewillbedisplayedintheRIWitness™Managersortedbydateandme.Figure 10-1 Combining parameter aributesFigure 10-2 Imaging screen showing operator and paent
Secon 1090Imaging10Ensurethatthereisalocaonsettostorecapturedimages.ThisissetinRIWitness™Manager.1. Click in the Main Menu tool bar.2. Click .3. SetthedesiredlepathwithintheImageStorageLocaoneld.Figure 10-3 Images captured shown in the RI Witness™ Manager screenFigure 10-4 Image storage locaonThis is available on both the work area screen and secondary Imaging screen.1. Log in using your operator name and PIN.2. Ensurethereisapaentwithanacve/opencycleintheworkarea.3. Press the  icon .
Secon 10Imaging9110This is available on both the work area screen and secondary Imaging screen.1. Login using your operator name and PIN.2. Ensurethereisapaentwithanacve/opencycleintheworkarea.3. Click the  icon .4. Eachvideorecordingcanlastforamaximumofonehour.Thiseliminatestheproblemofaccidentallyleavingthesowarerecordingandpossiblyrenderingthecomputerunusable.5. When freeze frame is set as keyboard shortcut, the live image recording can be paused. A second press of the shortcut key will restart the live image recording. 6. Press or click  to stop recording.Available on the Imaging screenThere are three ways to zoom into an area on the screen. 1. Placethemouseatapointonthescreen.Clickandholdtherightmousebuon,thenrelease.This will zoom to the preset zoom level into the spot where the mouse is placed. To zoom out, clickandholdtherightmousebuon.2. Place the mouse at a point on the screen and use the scroll wheel on the mouse (if available).This will give the user control over how much digital zoom is given. 3. Usingthetoolbarattheboomofthescreen,clickthe+ icon to zoom in incrementally. To zoom out by the same amount, click on the icon on the tool bar. The magnifying glass icon will zoom in and out by the preset zoom. Whilstusingdigitalzoom,athumbnailimageofthescreenwillappearinthetopleofthescreen.Atthetopofthispanelistheamountofmagnicaonyouareusing.Whenzoomedoutfully,thispanelwill not appear on the screen. Zooming in and zooming out can also be achieved using the foot pedal. Available on the Imaging screen1. Click  icon on the tool bar. 2. Click on the screen to select a start point for the measurement. 3. Clickonthescreenasecondmetoselectanendpointforthemeasurement.The ends of the line can be dragged to change the measurement. Linescanberemovedbyclickingthecrossinthecontextwindow.Duringlaseroperaon,linesareshownbutcannotbedragged/createdandnomeasurementswillbedisplayed. Lines and measurements are shown on an image when images are taken in Line Mode.
Secon 1092Imaging10Available on both the work area screen and secondary Imaging screen.1. Open 2. Unlocksengs.3. Press 4. Click on thetoallowtheseleconofcameras.Clickingontherequiredcamerain the list will change the live image to that of the selected camera. Available on both the work area screen and secondary Imaging screen.1. Open 2. Unlocksengs.3. Press 4. Tick (forcameradevicesthatsupporttheseopons).5. Tick (forcameradevicesthatsupporttheseopons).Available on the Imaging screen.1. Click the  icon from the Imaging screen. 2. Drag the Preset Zoom slider to the required zoom. Available on both the work area screen and secondary Imaging screen.1. Open 2. Unlocksengs.3. Press . 4. Clickthecorrespondingeditbuontoassignashortcut.5. Hold down the key or press the foot pedal to assign. 6. Click or press the  to save the shortcut. Oncetheshortcutsaresetup,presstheshortcutkeysorthecorrespondingfootpedaltoacvatethecommand.Available on the Imaging screen.1. Click the  on the tool bar. 2. Clicktherequiredobjecvemagnicaon.
Secon 10Imaging9310Available on the Imaging screen.1. Click on the tool bar. 2. Click the buon.3. Click Themagnicaonwillbethesameasthecurrentmagnicaon.4. Clicktherequiredobjecvetoeditobjecvemagnicaonandneadjustmentvalues.Available on the Imaging screen.1. Click on the tool bar. 2. Click the buon.3. Click Xnexttoanobjecve.Available on the Imaging screen1. Click on the tool bar. 2. Click the buontoopentheObjecveCalibraonpanel.3. Place an object of known dimensions (a stage micrometer is supplied with each system for this purpose)intheeldofview.4. EnsurethattheobjecveselectedinImagingmatchesthatbeingusedonthemicroscope.5. Thestagemicrometersuppliedmeasures100μmbetweenthelongerlines.Forbestaccuracy,posioneachendpointofthelineatexactlythesamerelaveposiononthescale,forexampleattherighthandedgeofeachvercalline.6. Drag the rulers to the point where the stage micrometer is going to be measured from and use theneadjustmentupanddownunltherulerscalematchesthestagemicrometer.1. Tocongureascreenasapaentdisplaysimplyselectthe for the desiredscreen.ThescreenwilldisplaytheRIlogounlthedisplayisturnedon.On OFigure 10-5 2. Toturnthepaentdisplayon/osothatitshowsthevideofromthecamera,clickthe or buoneitherontheworkareaoronthepaentdisplaypanelontheImagingdisplay.TheImagingdisplayshowsapreviewofwhatthepaentdisplaywillshow.SeeFigure10-5.3. Totakeasnapshotofthevideotouseonthepaentdisplay,pressthebuonontheworkareaorpaentdisplaypanel.4. To re-enable live streaming of the video, press .
Secon 10Imaging10Whenacurrentpaentisacvatedviatheworkarea,thepaentnameandIDwillappearonthePaentDisplay.Itwillonlybevisibleifthepaentdisplayisturnedon.SeeFigure10-6.5. Closingthemainwindowwillalsoclosethepaentdisplay.RIWitness™canberunalongsideandinconjunconwithSaturn™LaserSystems.PleaserefertotheSaturn™LaserSystemusermanualforfullinstrucons.Toaccesssengsandinformaonfromtheworkarea1. Open 2. Unlocksengs.3. DetailedinformaonabouttheSaturn™LaserSystemwillbedisplayed.4. Press  icon toenableBiopsyorMul-PulseModes.Figure 10-6 Informaon shown on paent display Figure 10-7 Saturn™ Laser System informaon
Secon 11Troubleshoong9511  Tags Reading IntermientlyorOnlyin Certain AreasRF noise Many devices in the lab can cause RF noise. ContactRIoraRIrepresentave.Antenna not tunedMetal objects may change antenna tuning. Posiontheantennawhereitwillbeused.Ifthe antenna that is not tuned is an auto tuning antennafollowtheinstruconsshownin“RFCalibraon”onpage19,otherwisecontactRIoraRIrepresentavetoretunetheantenna.Antenna not tunedMetal objects may change antenna tuning. Posiontheantennawhereitwillbeused.Ifthe antenna that is not tuned is an auto tuning antennafollowtheinstruconsshownin“RFCalibraon”onpage19,otherwisecontactRIoraRIrepresentavetoretunetheantenna.Looseconnecon Checkforanycablesunpluggedornotghtenedfully. Control unit / antenna / mulplexerfaulty ContactRIoraRIrepresentave.Tags Not ReadingBroken tag Checkthetagonadierentantenna.Tag not encryptedUse the . Non encrypted tags are shown in red.RF noise Many devices in the lab can cause RF noise. Contact RIoraRIrepresentave.WorkAreaconguraonUse the   to check that the number of antennas matches the number in the work area. Also check that the temperature controllerisspeciedcorrectly.Antenna not tunedMetalobjectsmaychangeantennatuning.Posionthe antenna where it will be used. If the antenna that is not tuned is an auto tuning antenna follow theinstruconsshownin“RFCalibraon”onpage19, otherwise contact RI or a RI representaveto retune the antenna.Looseconnecon Checkforanycablesunpluggedornotghtenedfully.ContactRIoraRIrepresentave.Control unit / antenna / mulplexerfaulty ContactRIoraRIrepresentave.
Secon 1196Troubleshoong11  Mulplexer / ControlUnit / Temp Controller MissingRI Witness™ WorkArea conguraonUse the   to check that the number of antennas matches the number in the work area. Also check that the temperature controllerisspeciedcorrectly.Looseconnecon Checkforanycablesunpluggedornotghtenedfully.Control unit / antenna / mulplexerfaultyContactRIoraRIrepresentaveDatabase MissingRI Witness™ WorkArea conguraonUse the  to check that database connecon is specied correctly. Contact your ITdepartment for assitstance.Looseconnecon Check for any unplugged or loose network cables. Replace with a known good cable.
Secon 12Temperature Calibraon9712Beforecheckingthetemperatureprole,itisimportanttoestablishifthereaderisaoneortwochannelreader.Thiscanbedonebyturningothecontrolboxandrestarng.Atthebeginningofthestart-upprocedurethecontrolboxscreenwillshowackoracrossnexttothechannelnumber(seeheatedcontrolboxdisplaywarnings).Ifthereaderisasinglechannelitwilldisplayacknexttochanneloneandacrossnexttochanneltwo,whilea2channelwilldisplayacknexttoboth.Thetemperaturegradientovertheheatedplateshouldnotexceed1.5°C.Inasinglechannelreaderthe temperature dierence between the centre of the glass window and the highest temperaturemeasuredawayfromthewindowshouldnotexceed3.0°C.Foradualchannelreaderthetemperaturedierencebetweenthecentreoftheglasswindowandthehighesttemperaturemeasuredawayfromthewindowshouldnotexceed1.5°C. Follow this guide to posion small amounts of thermal paste at each point to be measured.  Complete the form ‘RI Witness™ Heated Antenna Service Record’ for each heated reader. The latest formsareavailablebycontacngRI. Use a thermometer probe secured with masking tape to measure the temperature at each of the 14 points. Allow 3-5 minutes for the probe reading to stabilise at each measurement point. TheobjecveoftemperaturecalibraonistoensurethatthetemperatureindicatedbytheRIWitness™sowareiswithin0.2°Cofthetemperaturemeasuredonaheatedreadersurface. Werecommendusingathermometercalibratedat37°Cedwithasmallthermocoupleprobe,suchas the RI IVF Thermometer.Ensure that the work area setup is as it will be used, eg microscope light on, fan on, etc.Figure 12-1 Temperature prole template
Secon 1298Temperature Calibraon12 Switch the control unit ON. Make surethe controlunit RF powerisswitchedo toavoidinterferencewithathermometerreading.  Place a small amount of thermal compound 5cm to the right of the reader window. Posion athermometer probe in the compound and secure the probe with masking tape. Waitunlthethermometertemperaturestabilises.Thiscantakeupto45minutes. Compare the temperature shown in the work area soware with the thermometer reading. Adierencewithin+/-0.2°Cisacceptable. If the readings are outside the above requirement then click  to open the temperature oset sengs and make adjustments. Increase the oset to increase the displayed reading,decreasetheosettodecreaseit. Allowasmalldelayfortheosetchangetoregister.Figure 12-2
Secon 12Temperature Calibraon9912Figure 12-3 Touchkit : USB controller screen shot Thetouchscreencursorshouldaccuratelytracktheposionofanoperatornger.Ifaccuracyproblemsareexperiencedthenuseamousetoacvatethecalibraonulityandperformthefollowingchecklist.1. Double click the  desktop icon, then click  on the  tab. 2. The touch screen shows a blinking Xinthelowerlecorner.TouchandholdtheblinkingXunlitstopsblinkingandmovestothenextcornerposion.Repeatthetouchandholdforeachcornerofthescreen.Oncompleona“calibraonnished”messageisdisplayed.3. Touchthescreeninafewrandomlocaons.Repeatthecalibraonifthecursorisnotcorrectlyposioned.
Secon 13100Care and Maintenance13RIWitness™readersmaybecleanedwithasoclothandmilddetergent.Areadermaybeliedandreturnedtoitsoriginallocaon.Donotdisconnectthecablesaachedtoanyreader.Do not use solvents for cleaning.Do not disconnect readers.Donotchangetheposionofreaders.
Secon 14Repairs and Returns10114AssumingRIWitness™isregularlymaintainedandrounelyserviced,itshouldperformasrequiredforaminimumof7yearsconnualuse,aerwhichmewerecommendyouconsideritsreplacement.Shouldyounoceimpairedperformanceand/oranyissueswheresafetyiscompromised,orhaveanyotherconcernsduringtheuseofRIWitness™,seektheadviceofRIortheirauthorisedrepresentavepromptly. In the event that you have a problem with a RI instrument, please follow the procedure below to ensure promptaenon.1. Readthe‘Troubleshoong’secon.2. If you require any further help contact your distributor or RI directly. RI will try to resolve the problem as quickly as possible.If the product is no longer serviceable it must be sent back to RI to be destroyed in an environmentally safe way. Do not dispose of RI Witness™ products with ‘normal’ waste.1. ContactRItoobtainaReturnedMaterialsAuthorisaon(RMA)number. Goods will not be replacedorrefundedwithoutprioragreementandclearlystangtheRMAnumber.2. Pack the item carefully in its original packaging. RI will not accept responsibility for damage due toincorrectpackaging.Replacementitemsoraddionalrepairswillbeinvoiced.3. ClearlylabelthepackagewiththeRMAnumber,markthepackage“Urgent-ReturnedItemsForRepair”,andshiptotheaddressonthenextpage.Goodsshouldbeinsuredfortheirfullvalueduring shipping.   Tel:+44(0)1326372753Fax:+44(0)1326378783 E-mail: service@research-instruments.com Website: www.research-instruments.comIncompliancewiththeEuropeanMedicalDeviceDirecve93/42/EECasamended,itisyourdutytoinformRIifyoubelievethisdevicehas,ormayhave,causedorcontributedtothedeathofapaentoruserortoaseriousdeterioraonintheirstateofhealth.
Secon 14102Repairs and Returns14Thank you for purchasing a RI product. To help RI develop the best tools for ART, we rely on customer feedback.Ifyouhaveanysuggesonsforhowwecanimproveourproductsortheinformaonweprovide with them, please send them to feedback@research-instruments.com.Yourfeedbackwillhelpusdeveloptheproductandsupporngmaterialstomeetyourfutureneeds.Thank you.

Navigation menu